Stable MEF Raf1 FF/FF Knockin Cell Line

CAT.NOUNITPRICE
T61071x106 cells / 1.0 ml
$0.00

Specifications


DescriptionRaf is a crucial component of the Ras/Raf/MEK/ERK signalling pathway. This signalling pathway is essential for proper regulation of gene expression/cell cycle progression/apoptosis. Many cancers exhibit disregulation or abnormal activity of this pathway making it a potential therapeutic target. Stable MEF Raf1 FF/FF Knockin Cells were obtained from knock-in mouse embryos carrying inactive Raf-1FF/FF mutant.
SKUT6107
SpeciesMouse (M. musculus)
Tissue/Organ/Organ SystemEmbryo
Cell MorphologyFibroblast-like
ApplicationsFor Research Use Only
Seeding Density20,000 - 40,000 cells/cm2
Donor GenderFemale
Donor GenderFemale
Donor AgeEmbryonic
Cell TypeDrug Discovery Cell Lines
Growth PropertiesAdherent
Expression ProfileRaf1 Y340F Y341F inactive mutant. Vector: pZIPTEX; neomycin resistance
Preservation Protocol1. Freeze Medium: 90% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
Unit quantity1x106 cells / 1.0 ml
QC1) FACS; 2)Western blot; 3) Raf kinase cascade assay; 4) MBP kinase assay
Documents


Supporting Protocol
MSDS

    QC

      Other

      FAQs


      There are no FAQs for this product yet!
      References


      There are no references for this product yet!