Cumate-pLenti-Cloning-SV40-GFP
| Vector Name | Cumate-pLenti-Cloning-SV40-GFP |
| VectorType | Cumate Inducible Vector |
| Antibiotic Information | Bacterial: Kanamycin Mammalian: Puromycin |
| Sequencing Primers | Cumate SeqFP: 5'--GACTTTCCAAAATGTCGTAATAACC--3' SV40 SeqRP: 5'--TAGTCAGCCATGGGGCGGAGA--3' |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta ggcgttacat aacttacggt aaatggcccg
2461 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
2521 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
2581 cacttggcag tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac
2641 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg
2701 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc
2761 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
2821 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc
2881 gccccgttga cgcaaatggg caagcttgcc gggtcgaggt aggcgtgtac ggtgggaggc
2941 ctatataagc aaccggtata atacaaacag accagattgt ctgtttgttc aattgagtac
3001 ttacgtaggt accccagtgt ggtggcctgc aggtgaattc actagtaccg gtaggcctgt
3061 cgacgatatc gggcccgcgg ccgctggatc ctctagaggt gtggaaagtc cccaggctcc
3121 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
3181 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
3241 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
3301 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
3361 gagctattcc agaagtagtg aggaggcttt tttggaggcc tgtcgaggcc accatggaga
3421 tcgagtgccg catcaccggc accctgaacg gcgtggagtt cgagctggtg ggcggcggag
3481 agggcacccc caagcagggc cgcatgacca acaagatgaa gagcaccaaa ggcgccctga
3541 ccttcagccc ctacctgctg agccacgtga tgggctacgg cttctaccac ttcggcacct
3601 accccagcgg ctacgagaac cccttcctgc acgccatcaa caacggcggc tacaccaaca
3661 cccgcatcga gaagtacgag gacggcggcg tgctgcacgt gagcttcagc taccgctacg
3721 aggccggccg cgtgatcggc gacttcaagg tggtgggcac cggcttcccc gaggacagcg
3781 tgatcttcac cgacaagatc atccgcagca acgccaccgt ggagcacctg caccccatgg
3841 gcgataacgt gctggtgggc agcttcgccc gcaccttcag cctgcgcgac ggcggctact
3901 acagcttcgt ggtggacagc cacatgcact tcaagagcgc catccacccc agcatcctgc
3961 agaacggggg ccccatgttc gccttccgcc gcgtggagga gctgcacagc aacaccgagc
4021 tgggcatcgt ggagtaccag cacgccttca agacccccat cgccttcgcc agatcccgcg
4081 ctcagtcgtc caattctgcc gtggacggca ccgccggacc cggctccacc ggatctcgcg
4141 agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc cctatgaccg
4201 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
4261 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
4321 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
4381 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
4441 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
4501 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
4561 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
4621 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
4681 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
4741 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgaacgcgtt
4801 ccggaaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
4861 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
4921 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
4981 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
5041 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
5101 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
5161 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
5221 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
5281 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
5341 gtctcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctgtccg
5401 gatggaaggg ctaattcact cccaacgaat acaagatctg ctttttgctt gtactgggtc
5461 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
5521 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
5581 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
5641 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
5701 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
5761 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
5821 tgtatcttat catgtctggc atctatgtcg ggtgcggaga aagaggtaat gaaatggcat
5881 tatgggtatt atgggtctgc attaatgaat cggccaacga tcccggtgtg aaataccgca
5941 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
6001 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
6061 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
6121 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
6181 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
6241 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
6301 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
6361 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
6421 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
6481 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
6541 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
6601 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
6661 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
6721 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
6781 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
6841 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
6901 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
6961 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
7021 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
7081 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
7141 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
7201 taatagtttg cgcaacgttg ttgaaaaagg atcttcacct agatcctttt cacgtagaaa
7261 gccagtccgc agaaacggtg ctgaccccgg atgaatgtca gctactgggc tatctggaca
7321 agggaaaacg caagcgcaaa gagaaagcag gtagcttgca gtgggcttac atggcgatag
7381 ctagactggg cggttttatg gacagcaagc gaaccggaat tgccagctgg ggcgccctct
7441 ggtaaggttg ggaagccctg caaagtaaac tggatggctt tctcgccgcc aaggatctga
7501 tggcgcaggg gatcaagctc tgatcaagag acaggatgag gatcgtttcg catgattgaa
7561 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
7621 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
7681 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag
7741 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
7801 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
7861 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
7921 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
7981 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
8041 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat
8101 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
8161 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
8221 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
8281 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
8341 ttctgaattt tgttaaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
8401 cggcaacatc ccttataaat caaaagaata gaccgcgata gggttgagtg ttgttccagt
8461 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
8521 ctatcagggc gatggcccac tacgtgaacc atcacccaaa tcaagttttt tgcggtcgag
8581 gtgccgtaaa gctctaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
8641 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
8701 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgcgcgct taatgcgccg
8761 ctacagggcg cgtccattcg ccattcagga tcgaattaat tcttaattaa catcatcaat
8821 aatatacctt
LOCUS dna 8830 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
misc_binding 2605..2610
/dbxref="REBASE:NdeI"
misc_binding 3008..3013
/dbxref="REBASE:KpnI"
misc_binding 3035..3040
/dbxref="REBASE:EcoRI"
misc_binding 3065..3070
/dbxref="REBASE:EcoRV"
misc_binding 3077..3084
/dbxref="REBASE:NotI"
misc_binding 3086..3091
/dbxref="REBASE:BamHI"
misc_binding 3092..3097
/dbxref="REBASE:XbaI"
Promoter 3098..3366
/gene="SV40 prom"
Rep_Origin 3265..3342
/gene="SV40 origin"
misc_binding 3345..3357
/dbxref="REBASE:SfiI"
ORF 3414..4793
/sequence="ORF_3 rf(3)"
Marker 4194..4793
/gene="puro marker"
Other Gene 5403..5455
/gene="delta_U3 other"
Other Gene 5456..5636
/gene="HIV-1_5_LTR other"
Rep_Origin 6142..6761
/gene="pBR322 origin"
Promoter 7414..7463
/gene="NEOKAN prom"
misc_binding 7521..7526
/dbxref="REBASE:BclI"
ORF 7552..8346
/sequence="ORF_2 rf(1)"
Marker 7555..8343
/gene="NTP_II marker"
Rep_Origin 8451..8756
/gene="f1 origin"