pLenti-GIII-CMV-C-term-HA
| Vector Name | pLenti-GIII-CMV-C-term-HA |
| VectorType | Lentiviral Plasmid |
| Antibiotic Information | Bacterial: Kanamycin. Mammalian: Puromycin. |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
| Additional Information | The gene expression is driven by a CMV promoter, with a C-terminal HA Tag. Original Vector: 9776bp Vector without Insert Casette: 8071bp |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat
841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt
1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca
1801 aaattttcgg gtttttcgaa cctagggttc cgcgttacat aacttacggt aaatggcccg
1861 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
1921 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
1981 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
2041 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
2101 cagtacatct acgtttagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
2161 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
2221 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
2281 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
2341 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
2401 gaaccgagtt taaactccct atcagtgata gagatctccc tatcagtgat agagagctag
2461 cgatatcaac aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca
2521 atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa acacaacata
2581 tccagtcact atggcggccg cattaggcac cccaggcttt acactttatg cttccggctc
2641 gtataatgtg tggattttga gttaggatcc gtcgagattt tcaggagcta aggaagctaa
2701 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
2761 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
2821 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
2881 tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg
2941 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
3001 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
3061 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
3121 gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
3181 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
3241 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca
3301 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta
3361 aagatctgga tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt
3421 ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct
3481 atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat
3541 atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc
3601 gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg
3661 aaatgaacgg ctcttttgct gacgagaaca ggggctggtg aaatgcagtt taaggtttac
3721 acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac
3781 acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc
3841 tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc
3901 gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc
3961 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc
4021 cttatacaca gccagtctgc aggtcgacca tagtgactgg atatgttgtg ttttacagta
4081 ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta
4141 cgtttctcgt tcagctttct tgtacaaagt ggttgatatc ctcgagtacc catacgacgt
4201 cccagactac gcttgagttt aaacacgcgt ggtgtggaaa gtccccaggc tccccagcag
4261 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
4321 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
4381 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
4441 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
4501 tccagaagta gtgaggaggc ttttttggag gccatgaccg agtacaagcc cacggtgcgc
4561 ctcgccaccc gcgacgacgt ccctcgggcc gtacgcaccc tcgccgccgc gttcgccgac
4621 taccccgcca cgcgccacac cgtggacccg gaccgccaca tcgagcgggt caccgagctg
4681 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
4741 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
4801 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
4861 gaagggctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
4921 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
4981 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
5041 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
5101 acctggtgca tgacccgcaa gcccggtgcc tgaacgcgtt ccggaaatca acctctggat
5161 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
5221 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
5281 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
5341 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
5401 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
5461 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
5521 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
5581 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
5641 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtctcgcctt cgccctcaga
5701 cgagtcggat ctccctttgg gccgcctccc cgcctgtccg gatggaaggg ctaattcact
5761 cccaacgaat acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
5821 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
5881 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
5941 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
6001 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
6061 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
6121 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
6181 atctatgtcg ggtgcggaga aagaggtaat gaaatggcat tatgggtatt atgggtctgc
6241 attaatgaat cggccaacgg atgtcggtgt gaaataccgc acagatgcgt aaggagaaaa
6301 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
6361 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
6421 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
6481 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
6541 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
6601 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
6661 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
6721 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
6781 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
6841 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
6901 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
6961 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
7021 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
7081 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
7141 cgttaaggga ttttggccat gagattatca aaaaggatct tcacctagat ccttttcacg
7201 tagaaagcca gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc
7261 tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg
7321 cgatagctag actgggcggt tttatggaca gcaagcgaac cggaattgcc agctggggcg
7381 ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaagg
7441 atctgatggc gcaggggatc aagctctgat caagagacag gatgaggatc gtttcgcatg
7501 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
7561 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
7621 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa
7681 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
7741 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
7801 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
7861 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
7921 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
7981 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc
8041 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
8101 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
8161 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
8221 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
8281 gagttcttct gaattttgtt aaaatttttg ttaaatcagc tcatttttta accaataggc
8341 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
8401 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
8461 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
8521 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
8581 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
8641 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
8701 tgcgccgcta cagggcgcgt ccattcgcca ttcaggatcg aattaattct taattaacat
8761 catcaataat ataccttttt tggattgaag ccaatatgat aatgaggggg tggagtttgt
8821 gacgtggcgc ggggcgtggg aacggggcgg gtgacgtagt agtgtggcgg aagtgtgatg
8881 ttgcaagtgt ggcggaacac atgtaagcga cggatgtggc aaaagtgacg tttttggtgt
8941 gcgccggtgt acacaggaag tgacaatttt cgcgcggttt taggcggatg ttgtagtaaa
9001 tttgggcgta accgagtaag atttggccat tttcgcggga aaactgaata agaggaagtg
9061 aaatctgaat aattttgtgt tactcatagc gcgtaatact ctcatgagcg gatacatatt
9121 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
9181 acctgacgta actataacgg tcctaaggta gcgaaaatgt agtcttatgc aatactcttg
9241 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
9301 cgtgcatgcc gattggtgga agtaaggtgg tacgat
LOCUS dna 9336 bp
FEATURES Location/Qualifiers
Other Gene 109..289
/gene="HIV-1_5_LTR other"
Other Gene 400..444
/gene="HIV-1_psi_pack other"
misc_binding 486..491
/dbxref="REBASE:NruI"
Regulatory_Seq 951..1184
/gene="RRE reg"
ORF 994..1695
/sequence="ORF_1 rf(1)"
misc_binding 2005..2010
/dbxref="REBASE:NdeI"
Promoter 2279..2360
/gene="CMV prom"
Regulatory_Seq 2416..2455
/gene="tetO reg"
Other Gene 2469..2593
/gene="attR1 other"
Other Gene 2493..2593
/gene="attR2 other"
misc_binding 2594..2601
/dbxref="REBASE:NotI"
Marker 2702..3361
/gene="CAT marker"
ORF 2702..3361
/sequence="ORF_4 rf(2)"
misc_binding 2915..2920
/dbxref="REBASE:EcoRI"
Other Gene 3703..4008
/gene="ccdB other"
misc_binding 3784..3789
/dbxref="REBASE:SmaI"
misc_binding 3784..3789
/dbxref="REBASE:XmaI"
misc_binding 4037..4042
/dbxref="REBASE:PstI"
Other Gene 4049..4168
/gene="attR1 other"
Other Gene 4049..4149
/gene="attR2 other"
misc_binding 4181..4186
/dbxref="REBASE:XhoI"
Promoter 4231..4499
/gene="SV40 prom"
Rep_Origin 4398..4475
/gene="SV40 origin"
ORF 4465..5133
/sequence="ORF_2 rf(1)"
misc_binding 4478..4490
/dbxref="REBASE:SfiI"
Marker 4534..5133
/gene="puro marker"
Other Gene 5743..5795
/gene="delta_U3 other"
Other Gene 5796..5976
/gene="HIV-1_5_LTR other"
Rep_Origin 6483..7102
/gene="pBR322 origin"
Promoter 7360..7409
/gene="NEOKAN prom"
misc_binding 7467..7472
/dbxref="REBASE:BclI"
ORF 7498..8292
/sequence="ORF_3 rf(1)"
Marker 7501..8289
/gene="NTP_II marker"
Rep_Origin 8397..8703
/gene="f1 origin"
Other Gene 8758..8859
/gene="RITR other"
Other Gene 8758..8859
/gene="LITR other"
Other Gene 8939..9087
/gene="Encap other"
BASE COUNT 2399 a 2219 c 2484 g 2234 t 0 others
ORIGIN
1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat
841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt
1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca
1801 aaattttcgg gtttttcgaa cctagggttc cgcgttacat aacttacggt aaatggcccg
1861 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
1921 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
1981 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
2041 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
2101 cagtacatct acgtttagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
2161 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
2221 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
2281 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
2341 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
2401 gaaccgagtt taaactccct atcagtgata gagatctccc tatcagtgat agagagctag
2461 cgatatcaac aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca
2521 atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa acacaacata
2581 tccagtcact atggcggccg cattaggcac cccaggcttt acactttatg cttccggctc
2641 gtataatgtg tggattttga gttaggatcc gtcgagattt tcaggagcta aggaagctaa
2701 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
2761 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
2821 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
2881 tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg
2941 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
3001 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
3061 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
3121 gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
3181 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
3241 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca
3301 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta
3361 aagatctgga tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt
3421 ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct
3481 atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat
3541 atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc
3601 gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg
3661 aaatgaacgg ctcttttgct gacgagaaca ggggctggtg aaatgcagtt taaggtttac
3721 acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac
3781 acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc
3841 tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc
3901 gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc
3961 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc
4021 cttatacaca gccagtctgc aggtcgacca tagtgactgg atatgttgtg ttttacagta
4081 ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta
4141 cgtttctcgt tcagctttct tgtacaaagt ggttgatatc ctcgagtacc catacgacgt
4201 cccagactac gcttgagttt aaacacgcgt ggtgtggaaa gtccccaggc tccccagcag
4261 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
4321 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
4381 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
4441 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
4501 tccagaagta gtgaggaggc ttttttggag gccatgaccg agtacaagcc cacggtgcgc
4561 ctcgccaccc gcgacgacgt ccctcgggcc gtacgcaccc tcgccgccgc gttcgccgac
4621 taccccgcca cgcgccacac cgtggacccg gaccgccaca tcgagcgggt caccgagctg
4681 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
4741 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
4801 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
4861 gaagggctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
4921 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
4981 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
5041 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
5101 acctggtgca tgacccgcaa gcccggtgcc tgaacgcgtt ccggaaatca acctctggat
5161 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
5221 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
5281 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
5341 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
5401 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
5461 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
5521 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
5581 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
5641 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtctcgcctt cgccctcaga
5701 cgagtcggat ctccctttgg gccgcctccc cgcctgtccg gatggaaggg ctaattcact
5761 cccaacgaat acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
5821 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
5881 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
5941 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
6001 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
6061 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
6121 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
6181 atctatgtcg ggtgcggaga aagaggtaat gaaatggcat tatgggtatt atgggtctgc
6241 attaatgaat cggccaacgg atgtcggtgt gaaataccgc acagatgcgt aaggagaaaa
6301 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
6361 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
6421 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
6481 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
6541 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
6601 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
6661 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
6721 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
6781 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
6841 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
6901 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
6961 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
7021 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
7081 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
7141 cgttaaggga ttttggccat gagattatca aaaaggatct tcacctagat ccttttcacg
7201 tagaaagcca gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc
7261 tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg
7321 cgatagctag actgggcggt tttatggaca gcaagcgaac cggaattgcc agctggggcg
7381 ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaagg
7441 atctgatggc gcaggggatc aagctctgat caagagacag gatgaggatc gtttcgcatg
7501 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
7561 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
7621 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa
7681 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
7741 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
7801 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
7861 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
7921 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
7981 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc
8041 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
8101 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
8161 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
8221 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
8281 gagttcttct gaattttgtt aaaatttttg ttaaatcagc tcatttttta accaataggc
8341 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
8401 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
8461 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
8521 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
8581 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
8641 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
8701 tgcgccgcta cagggcgcgt ccattcgcca ttcaggatcg aattaattct taattaacat
8761 catcaataat ataccttttt tggattgaag ccaatatgat aatgaggggg tggagtttgt
8821 gacgtggcgc ggggcgtggg aacggggcgg gtgacgtagt agtgtggcgg aagtgtgatg
8881 ttgcaagtgt ggcggaacac atgtaagcga cggatgtggc aaaagtgacg tttttggtgt
8941 gcgccggtgt acacaggaag tgacaatttt cgcgcggttt taggcggatg ttgtagtaaa
9001 tttgggcgta accgagtaag atttggccat tttcgcggga aaactgaata agaggaagtg
9061 aaatctgaat aattttgtgt tactcatagc gcgtaatact ctcatgagcg gatacatatt
9121 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
9181 acctgacgta actataacgg tcctaaggta gcgaaaatgt agtcttatgc aatactcttg
9241 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
9301 cgtgcatgcc gattggtgga agtaaggtgg tacgat
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 cgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccactgaattg 60
GCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGACTTAAC
HIV-1_5_LTR other(109,289)>>>
|
61 ccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtctctctgg 120
GGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGAGAGACC
121 ttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcct 180
AATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGA
181 caataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggt 240
GTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCA
241 aactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccga 300
TTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCGCGGGCT
301 acagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactcggcttg 360
TGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAGCCGAAC
HIV-1_psi_pack other(400,444)>>>
|
361 ctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttga 420
GACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTTAAAACT
421 ctagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaa 480
GATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCCCCTCTT
NruI
|
481 ttagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaa 540
AATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATATTTAATT
541 aacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttag 600
TTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGGACAATC
601 aaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggat 660
TTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCTGTCCTA
661 cagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaagga 720
GTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAGTTTCCT
721 tagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagta 780
ATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGTTTTCAT
781 agaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgagggacat 840
TCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTCCCTGTA
841 tggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcaccc 900
ACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCATCGTGGG
RRE reg(951,1184)>>>
|
901 accaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttg 960
TGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCTCGAAAC
ORF_1 rf(1)(994,1695)>>>
|
961 ttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacg 1020
AAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGCGACTGC
1021 gtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggct 1080
CATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGACTCCCGA
1081 attgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggca 1140
TAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAGGTCCGT
1141 agaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgc 1200
TCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACCCCAACG
1201 tctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatct 1260
AGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTATTTAGA
1261 ctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaacaattac 1320
GACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTGTTAATG
1321 acaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaa 1380
TGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTACTTGTT
1381 gaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattgg 1440
CTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGTTTAACC
1441 ctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtt 1500
GACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCTTATCAA
1501 tttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcag 1560
AAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGCAAAGTC
1561 acccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtgga 1620
TGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTTCCACCT
1621 gagagagacagagacagatccattcgattagtgaacggatctcgacggtatcgaaagctt 1680
CTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCTTTCGAA
1681 gggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagt 1740
CCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTCTTATCA
1741 agacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaaaattca 1800
TCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTTTTAAGT
1801 aaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaatggcccg 1860
TTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTACCGGGC
1861 cctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccata 1920
GGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAAGGGTAT
1921 gtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcc 1980
CATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATTTGACGG
NdeI
|
1981 cacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgac 2040
GTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAGTTACTG
2041 ggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttgg 2100
CCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGATGAACC
2101 cagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagtacatca 2160
GTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCATGTAGT
2161 atgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtca 2220
TACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAACTGCAGT
CMV prom(2279,2360)>>>
|
2221 atgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccg 2280
TACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGTTGAGGC
2281 ccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctc 2340
GGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGTCTCGAG
2341 gtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaa 2400
CAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGGTATCTT
tetO reg(2416,2455)>>>
|
2401 gaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagagagctag 2460
CTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTCTCGATC
attR2 other(2493,2593)<<<
|
attR1 other(2469,2593)>>>
| |
2461 cgatatcaacaagtttgtacaaaaaagctgaacgagaaacgtaaaatgatataaatatca 2520
GCTATAGTTGTTCAAACATGTTTTTTCGACTTGCTCTTTGCATTTTACTATATTTATAGT
2521 atatattaaattagattttgcataaaaaacagactacataatactgtaaaacacaacata 2580
TATATAATTTAATCTAAAACGTATTTTTTGTCTGATGTATTATGACATTTTGTGTTGTAT
NotI
|
2581 tccagtcactatggcggccgcattaggcaccccaggctttacactttatgcttccggctc 2640
AGGTCAGTGATACCGCCGGCGTAATCCGTGGGGTCCGAAATGTGAAATACGAAGGCCGAG
2641 gtataatgtgtggattttgagttaggatccgtcgagattttcaggagctaaggaagctaa 2700
CATATTACACACCTAAAACTCAATCCTAGGCAGCTCTAAAAGTCCTCGATTCCTTCGATT
ORF_4 rf(2)(2702,3361)>>>
|
CAT marker(2702,3361)>>>
|
2701 aatggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaaga 2760
TTACCTCTTTTTTTAGTGACCTATATGGTGGCAACTATATAGGGTTACCGTAGCATTTCT
2761 acattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctgga 2820
TGTAAAACTCCGTAAAGTCAGTCAACGAGTTACATGGATATTGGTCTGGCAAGTCGACCT
2821 tattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttat 2880
ATAATGCCGGAAAAATTTCTGGCATTTCTTTTTATTCGTGTTCAAAATAGGCCGGAAATA
EcoRI
|
2881 tcacattcttgcccgcctgatgaatgctcatccggaattccgtatggcaatgaaagacgg 2940
AGTGTAAGAACGGGCGGACTACTTACGAGTAGGCCTTAAGGCATACCGTTACTTTCTGCC
2941 tgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactga 3000
ACTCGACCACTATACCCTATCACAAGTGGGAACAATGTGGCAAAAGGTACTCGTTTGACT
3001 aacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatata 3060
TTGCAAAAGTAGCGAGACCTCACTTATGGTGCTGCTAAAGGCCGTCAAAGATGTGTATAT
3061 ttcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattga 3120
AAGCGTTCTACACCGCACAATGCCACTTTTGGACCGGATAAAGGGATTTCCCAAATAACT
3121 gaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgt 3180
CTTATACAAAAAGCAGAGTCGGTTAGGGACCCACTCAAAGTGGTCAAAACTAAATTTGCA
3181 ggccaatatggacaacttcttcgcccccgttttcaccatgggcaaatattatacgcaagg 3240
CCGGTTATACCTGTTGAAGAAGCGGGGGCAAAAGTGGTACCCGTTTATAATATGCGTTCC
3241 cgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtttgtgatggcttcca 3300
GCTGTTCCACGACTACGGCGACCGCTAAGTCCAAGTAGTACGGCAAACACTACCGAAGGT
3301 tgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggcgta 3360
ACAGCCGTCTTACGAATTACTTAATGTTGTCATGACGCTACTCACCGTCCCGCCCCGCAT
3361 aagatctggatccggcttactaaaagccagataacagtatgcgtatttgcgcgctgattt 3420
TTCTAGACCTAGGCCGAATGATTTTCGGTCTATTGTCATACGCATAAACGCGCGACTAAA
3421 ttgcggtataagaatatatactgatatgtatacccgaagtatgtcaaaaagaggtatgct 3480
AACGCCATATTCTTATATATGACTATACATATGGGCTTCATACAGTTTTTCTCCATACGA
3481 atgaagcagcgtattacagtgacagttgacagcgacagctatcagttgctcaaggcatat 3540
TACTTCGTCGCATAATGTCACTGTCAACTGTCGCTGTCGATAGTCAACGAGTTCCGTATA
3541 atgatgtcaatatctccggtctggtaagcacaaccatgcagaatgaagcccgtcgtctgc 3600
TACTACAGTTATAGAGGCCAGACCATTCGTGTTGGTACGTCTTACTTCGGGCAGCAGACG
3601 gtgccgaacgctggaaagcggaaaatcaggaagggatggctgaggtcgcccggtttattg 3660
CACGGCTTGCGACCTTTCGCCTTTTAGTCCTTCCCTACCGACTCCAGCGGGCCAAATAAC
ccdB other(3703,4008)>>>
|
3661 aaatgaacggctcttttgctgacgagaacaggggctggtgaaatgcagtttaaggtttac 3720
TTTACTTGCCGAGAAAACGACTGCTCTTGTCCCCGACCACTTTACGTCAAATTCCAAATG
3721 acctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgac 3780
TGGATATTTTCTCTCTCGGCAATAGCAGACAAACACCTACATGTCTCACTATAATAACTG
SmaI
|
XmaI
| |
3781 acgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtc 3840
TGCGGGCCCGCTGCCTACCACTAGGGGGACCGGTCACGTGCAGACGACAGTCTATTTCAG
3841 tcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccacc 3900
AGGGCACTTGAAATGGGCCACCACGTATAGCCCCTACTTTCGACCGCGTACTACTGGTGG
3901 gatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgc 3960
CTATACCGGTCACACGGCCAGAGGCAATAGCCCCTTCTTCACCGACTAGAGTCGGTGGCG
3961 gaaaatgacatcaaaaacgccattaacctgatgttctggggaatataaatgtcaggctcc 4020
CTTTTACTGTAGTTTTTGCGGTAATTGGACTACAAGACCCCTTATATTTACAGTCCGAGG
attR2 other(4049,4149)>>>
|
PstI attR1 other(4049,4168)<<<
| |
4021 cttatacacagccagtctgcaggtcgaccatagtgactggatatgttgtgttttacagta 4080
GAATATGTGTCGGTCAGACGTCCAGCTGGTATCACTGACCTATACAACACAAAATGTCAT
4081 ttatgtagtctgttttttatgcaaaatctaatttaatatattgatatttatatcatttta 4140
AATACATCAGACAAAAAATACGTTTTAGATTAAATTATATAACTATAAATATAGTAAAAT
XhoI
|
4141 cgtttctcgttcagctttcttgtacaaagtggttgatatcctcgagtacccatacgacgt 4200
GCAAAGAGCAAGTCGAAAGAACATGTTTCACCAACTATAGGAGCTCATGGGTATGCTGCA
SV40 prom(4231,4499)>>>
|
4201 cccagactacgcttgagtttaaacacgcgtggtgtggaaagtccccaggctccccagcag 4260
GGGTCTGATGCGAACTCAAATTTGTGCGCACCACACCTTTCAGGGGTCCGAGGGGTCGTC
4261 gcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccag 4320
CGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTCCACACCTTTCAGGGGTC
4321 gctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcc 4380
CGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTATCAGG
SV40 origin(4398,4475)>>>
|
4381 cgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgcccc 4440
GCGGGGATTGAGGCGGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGG
SfiI
|
ORF_2 rf(1)(4465,5133)>>>
| |
4441 atggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctat 4500
TACCGACTGATTAAAAAAAATAAATACGTCTCCGGCTCCGGCGGAGCCGGAGACTCGATA
puro marker(4534,5133)>>>
|
4501 tccagaagtagtgaggaggcttttttggaggccatgaccgagtacaagcccacggtgcgc 4560
AGGTCTTCATCACTCCTCCGAAAAAACCTCCGGTACTGGCTCATGTTCGGGTGCCACGCG
4561 ctcgccacccgcgacgacgtccctcgggccgtacgcaccctcgccgccgcgttcgccgac 4620
GAGCGGTGGGCGCTGCTGCAGGGAGCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTG
4621 taccccgccacgcgccacaccgtggacccggaccgccacatcgagcgggtcaccgagctg 4680
ATGGGGCGGTGCGCGGTGTGGCACCTGGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGAC
4681 caagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgac 4740
GTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCTG
4741 ggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgcc 4800
CCGCGGCGCCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGG
4801 gagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatg 4860
CTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTAC
4861 gaagggctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggc 4920
CTTCCCGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCCG
4921 gtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggag 4980
CAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTC
4981 gcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctcccc 5040
CGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGG
5041 ttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgc 5100
AAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGGCGCG
5101 acctggtgcatgacccgcaagcccggtgcctgaacgcgttccggaaatcaacctctggat 5160
TGGACCACGTACTGGGCGTTCGGGCCACGGACTTGCGCAAGGCCTTTAGTTGGAGACCTA
5161 tacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgt 5220
ATGTTTTAAACACTTTCTAACTGACCATAAGAATTGATACAACGAGGAAAATGCGATACA
5221 ggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttc 5280
CCTATGCGACGAAATTACGGAAACATAGTACGATAACGAAGGGCATACCGAAAGTAAAAG
5281 tcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcagg 5340
AGGAGGAACATATTTAGGACCAACGACAGAGAAATACTCCTCAACACCGGGCAACAGTCC
5341 caacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgcc 5400
GTTGCACCGCACCACACGTGACACAAACGACTGCGTTGGGGGTGACCAACCCCGTAACGG
5401 accacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaa 5460
TGGTGGACAGTCGAGGAAAGGCCCTGAAAGCGAAAGGGGGAGGGATAACGGTGCCGCCTT
5461 ctcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaat 5520
GAGTAGCGGCGGACGGAACGGGCGACGACCTGTCCCCGAGCCGACAACCCGTGACTGTTA
5521 tccgtggtgttgtcggggaagctgacgtcctttccatggctgctcgcctgtgttgccacc 5580
AGGCACCACAACAGCCCCTTCGACTGCAGGAAAGGTACCGACGAGCGGACACAACGGTGG
5581 tggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggacctt 5640
ACCTAAGACGCGCCCTGCAGGAAGACGATGCAGGGAAGCCGGGAGTTAGGTCGCCTGGAA
5641 ccttcccgcggcctgctgccggctctgcggcctcttccgcgtctcgccttcgccctcaga 5700
GGAAGGGCGCCGGACGACGGCCGAGACGCCGGAGAAGGCGCAGAGCGGAAGCGGGAGTCT
delta_U3 other(5743,5795)>>>
|
5701 cgagtcggatctccctttgggccgcctccccgcctgtccggatggaagggctaattcact 5760
GCTCAGCCTAGAGGGAAACCCGGCGGAGGGGCGGACAGGCCTACCTTCCCGATTAAGTGA
HIV-1_5_LTR other(5796,5976)>>>
|
5761 cccaacgaatacaagatctgctttttgcttgtactgggtctctctggttagaccagatct 5820
GGGTTGCTTATGTTCTAGACGAAAAACGAACATGACCCAGAGAGACCAATCTGGTCTAGA
5821 gagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgc 5880
CTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCGAACG
5881 cttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccc 5940
GAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCATTGATCTCTAGGG
5941 tcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatcttatta 6000
AGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCATCATCAAGTACAGTAGAATAAT
6001 ttcagtatttataacttgcaaagaaatgaatatcagagagtgagaggaacttgtttattg 6060
AAGTCATAAATATTGAACGTTTCTTTACTTATAGTCTCTCACTCTCCTTGAACAAATAAC
6061 cagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttt 6120
GTCGAATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAA
6121 tttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggc 6180
AAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATAGTACAGACCG
6181 atctatgtcgggtgcggagaaagaggtaatgaaatggcattatgggtattatgggtctgc 6240
TAGATACAGCCCACGCCTCTTTCTCCATTACTTTACCGTAATACCCATAATACCCAGACG
6241 attaatgaatcggccaacggatgtcggtgtgaaataccgcacagatgcgtaaggagaaaa 6300
TAATTACTTAGCCGGTTGCCTACAGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTT
6301 taccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcgg 6360
ATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCC
6361 ctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggg 6420
GACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCC
6421 gataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaag 6480
CTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTC
pBR322 origin(6483,7102)<<<
|
6481 gccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcga 6540
CGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCT
6541 cgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccct 6600
GCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGA
6601 ggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcc 6660
CCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGG
6661 tttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcg 6720
AAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGC
6721 gtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgc 6780
CACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCG
6781 tgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgcca 6840
ACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGT
6841 ctggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagag 6900
GACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTC
6901 ttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgct 6960
AAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGA
6961 ctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaacc 7020
GACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGG
7021 accgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaagga 7080
TGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCT
7081 tctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactca 7140
AGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGT
7141 cgttaagggattttggccatgagattatcaaaaaggatcttcacctagatccttttcacg 7200
GCAATTCCCTAAAACCGGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAAGTGC
7201 tagaaagccagtccgcagaaacggtgctgaccccggatgaatgtcagctactgggctatc 7260
ATCTTTCGGTCAGGCGTCTTTGCCACGACTGGGGCCTACTTACAGTCGATGACCCGATAG
7261 tggacaagggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtgggcttacatgg 7320
ACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTACC
NEOKAN prom(7360,7409)>>>
|
7321 cgatagctagactgggcggttttatggacagcaagcgaaccggaattgccagctggggcg 7380
GCTATCGATCTGACCCGCCAAAATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGC
7381 ccctctggtaaggttgggaagccctgcaaagtaaactggatggctttcttgccgccaagg 7440
GGGAGACCATTCCAACCCTTCGGGACGTTTCATTTGACCTACCGAAAGAACGGCGGTTCC
BclI ORF_3 rf(1)(7498,8292)>>>
| |
7441 atctgatggcgcaggggatcaagctctgatcaagagacaggatgaggatcgtttcgcatg 7500
TAGACTACCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTAC
NTP_II marker(7501,8289)>>>
|
7501 attgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggc 7560
TAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCG
7561 tatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcg 7620
ATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGC
7621 caggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaa 7680
GTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTT
7681 gacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctc 7740
CTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAG
7741 gacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggat 7800
CTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTA
7801 ctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcgg 7860
GAGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCC
7861 cggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatc 7920
GCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAG
7921 gagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagag 7980
CTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTC
7981 catcaggggctcgcgccagccgaactgttcgccaggctcaaggcgagcatgcccgacggc 8040
GTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCG
8041 gaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggc 8100
CTCCTAGAGCAGCACTGGGTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCG
8101 cgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacata 8160
GCGAAAAGACCTAAGTAGCTGACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTAT
8161 gcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctc 8220
CGCAACCGATGGGCACTATAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAG
8221 gtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgac 8280
CACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTG
8281 gagttcttctgaattttgttaaaatttttgttaaatcagctcattttttaaccaataggc 8340
CTCAAGAAGACTTAAAACAATTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCG
f1 origin(8397,8703)<<<
|
8341 cgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgt 8400
GCTTTAGCCGTTTTAGGGAATATTTAGTTTTCTTATCTGGCTCTATCCCAACTCACAACA
8401 tccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaa 8460
AGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTT
8461 aaccgtctatcagggcgatggcccactacgtgaaccatcaccctaatcaagttttttggg 8520
TTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGGATTAGTTCAAAAAACCC
8521 gtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttg 8580
CAGCTCCACGGCATTTCGTGATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAAC
8581 acggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgc 8640
TGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCG
8641 tagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaa 8700
ATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCATTGGTGGTGTGGGCGGCGCGAATT
LITR other(8758,8859)>>>
|
RITR other(8758,8859)<<<
|
8701 tgcgccgctacagggcgcgtccattcgccattcaggatcgaattaattcttaattaacat 8760
ACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGTA
8761 catcaataatataccttttttggattgaagccaatatgataatgagggggtggagtttgt 8820
GTAGTTATTATATGGAAAAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACA
8821 gacgtggcgcggggcgtgggaacggggcgggtgacgtagtagtgtggcggaagtgtgatg 8880
CTGCACCGCGCCCCGCACCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTAC
Encap other(8939,9087)>>>
|
8881 ttgcaagtgtggcggaacacatgtaagcgacggatgtggcaaaagtgacgtttttggtgt 8940
AACGTTCACACCGCCTTGTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACA
8941 gcgccggtgtacacaggaagtgacaattttcgcgcggttttaggcggatgttgtagtaaa 9000
CGCGGCCACATGTGTCCTTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTT
9001 tttgggcgtaaccgagtaagatttggccattttcgcgggaaaactgaataagaggaagtg 9060
AAACCCGCATTGGCTCATTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCAC
9061 aaatctgaataattttgtgttactcatagcgcgtaatactctcatgagcggatacatatt 9120
TTTAGACTTATTAAAACACAATGAGTATCGCGCATTATGAGAGTACTCGCCTATGTATAA
9121 tgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgcc 9180
ACTTACATAAATCTTTTTATTTGTTTATCCCCAAGGCGCGTGTAAAGGGGCTTTTCACGG
9181 acctgacgtaactataacggtcctaaggtagcgaaaatgtagtcttatgcaatactcttg 9240
TGGACTGCATTGATATTGCCAGGATTCCATCGCTTTTACATCAGAATACGTTATGAGAAC
9241 tagtcttgcaacatggtaacgatgagttagcaacatgccttacaaggagagaaaaagcac 9300
ATCAGAACGTTGTACCATTGCTACTCAATCGTTGTACGGAATGTTCCTCTCTTTTTCGTG
9301 cgtgcatgccgattggtggaagtaaggtggtacgat 9336
GCACGTACGGCTAACCACCTTCATTCCACCATGCTA
LOCUS dna 9336 bp
FEATURES Location/Qualifiers
Other Gene 109..289
/gene="HIV-1_5_LTR other"
Other Gene 400..444
/gene="HIV-1_psi_pack other"
misc_binding 486..491
/dbxref="REBASE:NruI"
Regulatory_Seq 951..1184
/gene="RRE reg"
ORF 994..1695
/sequence="ORF_1 rf(1)"
misc_binding 2005..2010
/dbxref="REBASE:NdeI"
Promoter 2279..2360
/gene="CMV prom"
Regulatory_Seq 2416..2455
/gene="tetO reg"
Other Gene 2469..2593
/gene="attR1 other"
Other Gene 2493..2593
/gene="attR2 other"
misc_binding 2594..2601
/dbxref="REBASE:NotI"
Marker 2702..3361
/gene="CAT marker"
ORF 2702..3361
/sequence="ORF_4 rf(2)"
misc_binding 2915..2920
/dbxref="REBASE:EcoRI"
Other Gene 3703..4008
/gene="ccdB other"
misc_binding 3784..3789
/dbxref="REBASE:SmaI"
misc_binding 3784..3789
/dbxref="REBASE:XmaI"
misc_binding 4037..4042
/dbxref="REBASE:PstI"
Other Gene 4049..4168
/gene="attR1 other"
Other Gene 4049..4149
/gene="attR2 other"
misc_binding 4181..4186
/dbxref="REBASE:XhoI"
Promoter 4231..4499
/gene="SV40 prom"
Rep_Origin 4398..4475
/gene="SV40 origin"
ORF 4465..5133
/sequence="ORF_2 rf(1)"
misc_binding 4478..4490
/dbxref="REBASE:SfiI"
Marker 4534..5133
/gene="puro marker"
Other Gene 5743..5795
/gene="delta_U3 other"
Other Gene 5796..5976
/gene="HIV-1_5_LTR other"
Rep_Origin 6483..7102
/gene="pBR322 origin"
Promoter 7360..7409
/gene="NEOKAN prom"
misc_binding 7467..7472
/dbxref="REBASE:BclI"
ORF 7498..8292
/sequence="ORF_3 rf(1)"
Marker 7501..8289
/gene="NTP_II marker"
Rep_Origin 8397..8703
/gene="f1 origin"
Other Gene 8758..8859
/gene="RITR other"
Other Gene 8758..8859
/gene="LITR other"
Other Gene 8939..9087
/gene="Encap other"
BASE COUNT 2399 a 2219 c 2484 g 2234 t 0 others
