pRRL

                            LOCUS       pRRL-cPPT-PGK-GFP-W 7384 bp DNA SYN 27-Aug-2007
DEFINITION  pRRL-cPPT-PGK-GFP-W
ACCESSION    
KEYWORDS    
SOURCE    
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..7384
                     /organism="pRRL-cPPT-PGK-GFP-W"
                     /mol_type="other DNA"
     misc_feature    236..416
                     /label="HIV-1_5_LTR"
     misc_feature    236..416
                     /label="truncHIV-1_3_LTR"
     misc_feature    524..568
                     /label="HIV-1_psi_pack"
     misc_feature    1078..1311
                     /label="RRE"
     CDS             956..2164
                     /label="ORF frame 2"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRLTFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFIDHETSLEKLD
                     IEFPRGWGCAFSKAALGLRRDAAALGVVPGNAAAPTLGLAHSSRPFAASPGSSPLPLW
                     APRRRFLLRP*"
     misc_feature    1811..1826
                     /label="cPPT"
     CDS             complement(1938..2546)
                     /label="ORF frame 3"
                     /translation="MGTTPVNSSSPLLTMVATGGSPWGERSVIRSTREPTADVRSGGL
                     QNAEHRAGRNRAHTTAPHPASRTAPSRPLLSARPAEQPLLATAHRGRRAAIAPWRCPS
                     ARVLVRRAASVCHVRHAANRKEPSRLRGGAGSVAGGPTRVAAKIRVTLRTDVKNVRDP
                     GSAPLRFPEPRPEQPRPCANPGLPWKRRNPNPVGIRYQASRG*"
     misc_feature    complement(2548..2569)
                     /label="EGFP_N_primer"
     CDS             2263..3222
                     /label="EGFP"
                     /gene="EGFP"
                     /note="ORF frame 1"
                     /translation="MAARRPRWAVANSGCSAGRAESSGREGAVREAGCGAVVWALFLP
                     ARCSAFCKPPERTSAVGSLVDRITDLSPQGDPPVATMVSKGEELFTGVVPILVELDGD
                     VNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMK
                     QHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNIL
                     GHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLL
                     PDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*"
     gene            2503..3219
                     /label="EGFP"
                     /gene="EGFP"
     misc_feature    3156..3177
                     /label="EGFP_C_primer"
     misc_feature    3240..3815
                     /label="WPRE"
     misc_feature    3896..3911
                     /label="cPPT"
     misc_feature    3896..3917
                     /label="U3PPT"
     misc_feature    3913..3965
                     /label="delta_U3"
     misc_feature    3966..4146
                     /label="HIV-1_5_LTR"
     misc_feature    3966..4146
                     /label="truncHIV-1_3_LTR"
     terminator      4221..4340
                     /label="SV40_PA_terminator"
     misc_feature    4309..4328
                     /label="EBV_rev_primer"
     rep_origin      4358..4435
                     /label="SV40_origin"
     misc_feature    4420..4439
                     /label="SV40pro_F_primer"
     promoter        complement(4526..4544)
                     /label="T7_promoter"
     misc_feature    complement(4554..4570)
                     /label="M13_forward20_primer"
     misc_feature    complement(4563..4585)
                     /label="M13_pUC_fwd_primer"
     misc_feature    4563..4706
                     /label="lacZ_a"
     rep_origin      4732..5038
                     /label="f1_origin"
     promoter        5234..5262
                     /label="AmpR_promoter"
     CDS             5304..6164
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /note="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     gene            5304..6164
                     /label="Ampicillin"
                     /gene="Ampicillin"
     rep_origin      6319..6938
                     /label="pBR322_origin"
     promoter        7247..7276
                     /label="lac_promoter"
     misc_feature    7290..7312
                     /label="M13_pUC_rev_primer"
     misc_feature    7311..7329
                     /label="M13_reverse_primer"
     promoter        7346..7365
                     /label="T3_promoter"
ORIGIN
    1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
   61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
  121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
  181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacaa taaacgggtc
  241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
  301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
  361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
  421 cgcccgaaca gggacctgaa agcgaaaggg aaaccagagc tctctcgacg caggactcgg
  481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
  541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
  601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
  661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
  721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
  781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
  841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
  901 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
  961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
 1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
 1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cctcaatgac
 1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
 1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
 1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
 1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
 1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
 1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
 1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
 1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
 1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
 1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
 1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
 1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
 1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
 1921 ttttatcgat cacgagacta gcctcgagaa gcttgatatc gaattcccac ggggttgggg
 1981 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
 2041 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
 2101 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
 2161 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
 2221 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
 2281 cgatgggctg tggccaatag cggctgctca gcggggcgcg ccgagagcag cggccgggaa
 2341 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
 2401 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga
 2461 atcaccgacc tctctcccca gggggatcca ccggtcgcca ccatggtgag caagggcgag
 2521 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
 2581 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
 2641 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
 2701 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
 2761 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
 2821 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
 2881 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
 2941 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
 3001 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
 3061 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
 3121 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
 3181 gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg cgtcgacaat
 3241 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
 3301 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
 3361 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
 3421 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
 3481 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
 3541 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
 3601 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
 3661 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
 3721 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
 3781 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattcgagct
 3841 cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
 3901 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
 3961 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
 4021 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
 4081 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
 4141 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
 4201 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
 4261 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
 4321 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccag
 4381 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
 4441 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
 4501 ttgcgtcgag acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
 4561 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
 4621 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
 4681 caacagttgc gcagcctgaa tggcgaatgg cgcgacgcgc cctgtagcgg cgcattaagc
 4741 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
 4801 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
 4861 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
 4921 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
 4981 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
 5041 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
 5101 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
 5161 tttacaattt cccaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
 5221 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
 5281 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
 5341 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
 5401 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
 5461 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
 5521 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
 5581 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
 5641 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
 5701 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
 5761 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
 5821 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
 5881 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
 5941 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
 6001 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
 6061 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
 6121 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
 6181 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
 6241 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
 6301 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
 6361 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
 6421 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
 6481 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
 6541 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
 6601 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
 6661 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
 6721 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
 6781 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
 6841 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
 6901 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
 6961 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
 7021 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
 7081 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
 7141 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
 7201 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
 7261 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
 7321 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
 7381 tgca
//