Zebrafish Testicular Feeder Cells (ZtA6-4)

CAT.NOUNITPRICE
T26031x106 cells/ml
$0.00

Specifications


DescriptionZtA6 cells are derived from spontaneous tumor-like hypertropheied testis isolated from albino-type (alb-1/alb-1) zebrafish. Clones were analyzed for the expression of the Sertoli cell marker (Sox9a), the germ cell marker (Vas), and the Wilm’s tumor suppressor marker (WT1). 12 clones were derived and have distinctive properties and are available at abm.

ZtA6-4 cells are clone 4 which has high phagocytic ability compared to the other clones derived from the ZtA6 cell line. The expression of Sox9a and Vas were observed, but WT1 expression was absent. It is able to support the differentiation of germ cells into functional sperm for in vitro fertilization, thus is a suitable tool for research in in vitro fertilization and vertebrate spermatogenesis.
SKUT2603
SpeciesZebrafish (D. rerio)
Species descriptionAlbino-type (alb-1/alb-1) zebrafish (Danio rerio)
Tissue/Organ/Organ SystemReproductive
Cell MorphologyEpithelial-like, Fibroblast-like
ApplicationsFor Research Use Only.
Cell TypeFeeder Cells
Growth PropertiesAdherent
Propagation RequirementsGrow cells in T25 gelatin-coated flasks (TM063) with the following conditions. The base medium for this cell line is L-15 medium (ThermoFisher Scientific). To make the completed growth medium, add the following components to the base medium at the following final concentrations: 5 IU/ml human chorionic gonadotropin (Sigma), 2 IU/ml pregnant mare’s serum gonadotropin (Sigma), 0.2 mg/ml L-arginine (Gibco BRL), 0.02 mg/ml L-aspartic acid (Gibco BRL), 0.015 mg/ml L-histidine (Gibco BRL), 0.0725 mg/ml L-lysine HCl (Gibco BRL), 0.02 mg/ml L-proline (Gibco BRL), 0.5% bovine serum albumin (5% w/v stock solution, BSA fraction V, Sigma), 1% Hepes (Sigma), fetal bovine serum (TM999) to a final concentration of 3%, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Atmosphere: air: 100%, Temperature: 28.0°C.

To prepare as feeder cells: Treat with 10 µg/ml mitomycin C in L-15 for 5 hours before plating for use as feeder cells for further experimentation.
Unit quantity1x106 cells/ml
QC1) Phagocytic activity was analyzed via the ability to internalize polystyrene beads;
2) RT-PCR was used to assess the presence or absence of WT1, Vas, and Sox9a markers;
3) Functionality test was performed to determine the ability to support male germ cells when co-cultured as feeder cells.
Storage Condition-180°C
Shipping ConditionsOn Dry Ice
Disclaimer

1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.

2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.

6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."

CautionFor Research Use Only.
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          References


          1
          • Kurita, K., & Sakai, N. (2004). Functionally distinctive testicular cell lines of zebrafish to support male germ cell development. Molecular reproduction and development, 67(4), 430–438. https://doi.org/10.1002/mrd.20035