Custom CRISPR Multiplex sgRNA Vectors
abm’s Custom CRISPR Multiplex sgRNA system allows for optimal expression of multiple sgRNAs from alternating U6 and H1 RNA pol III promoters on a single lentiviral vector (for other vector options please inquire). This allows for expression of multiple sgRNAs targeting a single gene or multiple sgRNAs targeting multiple different genes. sgRNAs may be designed for use with any Cas9 variant, which is expressed from a separate vector. Together, the Multiplex sgRNA Vectors expand the CRISPR toolkit allowing for highly diverse gene editing applications. To get started on your custom project, simply click the Inquire button below.
Applications
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- Gene activation using dCas9-VPR
- Gene repression using dCas9-KRAB
- Epigenetic gene activation using dCas9-TET1CD
- Epigenetic gene repression using dCas9-DNMT3A
- Gene Knockout using spCas9 or saCas9
Service Details
Cloning Services
| sgRNA Service (for spCas9) | No. of sgRNAs | Quantity | Cat. No. | Price |
|---|---|---|---|---|
| Custom CRISPR Dual sgRNA Lentivector (for spCas9) | 2 | 1.0 ug | C420 | $395.00 |
| Custom CRISPR Triple sgRNA Lentivector (for spCas9) | 3 | 1.0 ug | C421 | $595.00 |
| Custom CRISPR Quad sgRNA Lentivector (for spCas9) | 4 | 1.0 ug | C422 | $795.00 |
| Custom CRISPR Multiplex sgRNA Lentivector (for spCas9) | >4 | 1.0 ug | C423 | Inquire |
| sgRNA Service (for saCas9) | No. of sgRNAs | Quantity | Cat. No. | Price |
|---|---|---|---|---|
| Custom CRISPR Dual sgRNA Lentivector (for saCas9) | 2 | 1.0 ug | C511 | $395.00 |
| Custom CRISPR Triple sgRNA Lentivector (for saCas9) | 3 | 1.0 ug | C512 | $595.00 |
| Custom CRISPR Quad sgRNA Lentivector (for saCas9) | 4 | 1.0 ug | C513 | $795.00 |
| Custom CRISPR Multiplex sgRNA Lentivector (for saCas9) | >4 | 1.0 ug | C514 | Inquire |
Lentivirus Packaging Services
| Service | Unit | Titer | Purity | Cat. No. | Lead Time | Price |
|---|---|---|---|---|---|---|
| Standard Titer Custom Recombinant Lentivirus Packaging | 4 x 500 μl | 107 IU/ml | Research grade | LV001-a | 2-3 weeks | $199 |
| Medium Titer Custom Recombinant Lentivirus Packaging | 4 x 250 µl | 108 IU/ml | Research grade | LV001-b | 2-3 weeks | $449 |
| High Titer Custom Recombinant Lentivirus Packaging | 10 x 100 µl | 109 IU/ml | Research grade | LV001-c | 2-3 weeks | $749 |
| Ultra-High Titer Custom Recombinant Lentivirus Packaging | 10 x 100 µl | 1010 IU/ml | Research grade | LV001-d | 2-3 weeks | $1049 |
| Custom Lentivirus Aliquoting | Up to 10 Vials | - | - | C507 | - | $5.00 |
Additional Info
Related Products
Documents
FAQs
| What promoter(s) do your sgRNA and All-in-One lentivectors have? |
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sgRNAs are expressed from the U6 promoter and All-in-One lentivectors and lentiviruses express Cas9 from the SFFV promoter.
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| What is the approximate time frame to generate CRISPR lentiviral constructs? |
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The typical lead time is around 1-3 weeks for CRISPR lentivectors and 4-6 weeks for lentiviruses. Please inquire for a more accurate lead time.
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| How should I store my lentivirus? |
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Aliquots should be made for the lentivirus and stored at -70 degrees Celsius.
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| If a high-titer custom lentivirus was ordered, do you do the lentiviral titration after a freeze-thaw or straight after production before the freeze-down step? |
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We do the titration after aliquoting and freeze-down. Thus, the titer should be accurate for the customer after they thaw the finished product for the first time.
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| Which virus has your lentivirus expression system been derived from? Is it HIV? |
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Our lentivirus expression system is derived from Human HIV-1 Virus. It employs third generation self-inactivating recombinant lentiviral vectors with enhanced biosafety features and minimal relation to wild-type Human HIV-1 Virus.
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| How do you verify the titer? |
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We use our LV900 series - qPCR Lentivirus Titration(Titer) Kit. This kit quantifies a proprietary region of the lentiviral 5’-LTR.
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| What is the packaging capacity for Lentivirus? |
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The maximum insert size is <9 kb between the 5'LTR to 3'LTR.
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Citations
| 01 | Metzger D. et al. "Host Cell Transcriptional Tuning with CRISPR/dCas9 to Mitigate the Effects of Toxin Exposure." ACS Synth. Biol. (2022) 11, 3657-3668. doi: 10.1021/acssynbio.2c00214 |
| 02 | Shirasaki, T. et al. "Nonlytic Quasi-Enveloped Hepatovirus Release Is Facilitated by pX Protein INteraction with the E3 Ubiquitin Ligase ITCH." J. Virol. (2022) 96(21). doi: 10.1128/jvi.01195-22 |