dCas9-DNMT3A Lentivirus
| Cat. No. | K092 |
| Name | dCas9-DNMT3A Lentivirus |
| Unit | 3 x 250 µl |
| Description |
Lentivirus expressing dCas9 (D10A and H840A) fused to the DNA methyltransferase (DNMT3A) domain. The DNMT3A domain facilitates the process of methylation leading to transcriptional down-regulation. By using specific sgRNAs, the dCas9-DNMT3A can be targeted to a genomic region allowing for epigenetic control over the expression of almost any gene of interest |
| Applications |
CRISPR epigenetic modification. |
| Titer | 107 IU/mL |
| Storage Condition | Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the viruses at -80°C in small aliquots to avoid repeated freeze-thaw cycles. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. K092 |
Print/Download Datasheet
| Where can I learn more about CRISPR dCas9 applications and genome regulation? | |
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You can check out our Learning Resources or watch our Youtube Video on dCas9 regulation.
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| Do I need to provide my own sgRNA? | |
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Yes, this product only expresses the Cas9 or Cas-related protein. We offer genome-wide sgRNA only expressing vectors for CRISPR gene knockout (spCas9 and saCas9) and CRISPR gene activation and repression which are available through the Customize Vector option. Similarly, abm also offers Custom CRISPR Vectors and Viruses, please inquire with us to get started on your CRISPR project.
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| How can I validate successful DNA methylation or demethylation? | |
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Common methods include: bisulfite sequencing, pyrosequencing, methylation-specific PCR, or methylation-sensitive restriction assays, alongside transcriptional expression analysis of the targeted gene.
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