dCas9-KRAB Lentivirus

Cat. No.
4 x 500ul
Cat. No. K204
Name dCas9-KRAB Lentivirus
Unit 4 x 500ul

dCas9-KRAB is used for CRISPR gene repression (CRISPRi). The Cas9 Double Mutant (dCas9) has changes at amino acid positions D10A and H840A which completely inactivate both the nuclease and nickase activities. In this construct, dCas9 carries a C-terminal Krüppel associated box (KRAB) domain. By targeting dCas9-KRAB to a promoter region, users will be able to specifically repress expression of virtually any gene of interest. This dCas9-KRAB lentivirus can be transduced for high efficiency stable integration of dCas9-KRAB into host cells. Use this dCas9-KRAB lentivirus in conjunction with a CRISPRi sgRNA lentivirus designed specifically for CRISPR repression.

Vector pLenti-EF1a-dCas9-KRAB
Titer 107 IU/mL
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. K204
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