dCas9-TET1CD Lentivirus

CAT.NOUNITPRICE
K090300 µl
$695.00

Specifications


Description

This construct consists of the catalytic domain of Ten-Eleven Translocation dioxygenase 1 (TET1CD) fused to the C-terminus of Cas9 Double Mutant (dCas9). The Cas9 Double Mutant has changes at amino acid positions D10A and H840A which completely inactivate both nuclease and nickase activities; whereas the TET1 domain facilitates the process of demethylation leading to transcriptional up-regulation. By using specific sgRNAs, the dCas9-TET1CD can be targeted to a promoter region allowing for epigenetic control over the expression of almost any gene of interest.

SKUK090
SystemLentivirus
Vector Map

pLenti-EF1a-dCas9-TET1CD

Unit quantity300 µl
Titer107 IU/ml
PromoterEF1a
Cas TypeNull Mutant
Cas OriginspCas9
Cas9 ExpressionConstitutive Cas9
Shipping Conditions

Shipped with dry ice

FormatVirus
AppearanceLiquid
Quality ControlRestriction Enzyme Digest and Sequencing
Shelf Life1 year (when at -80°C or below in a non-frost free freezer)
CautionThis product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.
Documents


Supporting Protocol

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