CRISPRa dCas9-VPR Lentivirus
| Cat. No. | K098 |
| Name | CRISPRa dCas9-VPR Lentivirus |
| Unit | 3 x 250 µl |
| Description |
Lentivirus expressing dCas9 (D10A and H840A) fused to the VPR activator complex (VP64, p65, Rta), which enhances gene expression by recruiting transcription factors. Unlike the dCas9-SAM system, dCas9-VPR doesn't require MS2-modified sgRNAs, simplifying delivery with a single fusion protein encoded in one vector. By using specific sgRNAs, the dCas9-VPR can be targeted to a promoter region allowing for activation of almost any gene of interest. |
| Applications |
CRISPR gene activation. |
| Titer | 108 IU/ml |
| Storage Condition | Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the viruses at -80°C in small aliquots to avoid repeated freeze-thaw cycles. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. K098 |
| Where can I learn more about CRISPR dCas9 applications and genome regulation? | |
|
You can check out our Learning Resources or watch our Youtube Video on dCas9 regulation.
|
| Do you offer sgRNA expressing vectors for CRISPR activation and repression? | |
|
Yes, abm offers genome-wide activation and repression sgRNAs. Check out our full catalogue here.
|
| What are the advantages of the dCas9-VPR system over dCas9-SAM? | |
|
dCas9-VPR system is advantageous over dCas9-SAM because it is a single fusion protein (dCas9 linked to VP64, p65, and Rta), making it simpler to use, easier to clone, and more practical for delivery – especially in size-limited systems like AAV. Unlike SAM, which requires multiple components (dCas9-VP64, modified sgRNA with MS2 loops, and MS2-p65-HSF1), VPR reduces system complexity and variability in expression. While SAM can sometimes provide stronger gene activation, VPR offers a more streamlined and robust approach for transcriptional activation, particularly in in vivo or therapeutic applications. |
| Do I need to provide my own sgRNA? | |
|
Yes, this product only expresses the Cas9 or Cas-related protein. We offer genome-wide sgRNA only expressing vectors for CRISPR gene knockout (spCas9 and saCas9) and CRISPR gene activation and repression which are available through the Customize Vector option. Similarly, abm also offers Custom CRISPR Vectors and Viruses, please inquire with us to get started on your CRISPR project.
|