Cas9 Nuclease GFP NLS Protein (High Concentration)

Cat. No.
K148
Unit
47µg (250pmol/ 25µL)
Price
$162.00
Cat. No. K148
Name Cas9 Nuclease GFP NLS Protein (High Concentration)
Unit 47µg (250pmol/ 25µL)
Category Molecular Biology Enzymes and Kits
Description

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement.


The Cas9 nuclease serves to unwind the genomic DNA duplex next to conserved protospacer adjacent motifs (PAMs) and homes in on its target sequence, which is recognized by a complementary single-guide RNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene. Alternatively, by supplying a suitable repair template, virtually any desired point mutation can be introduced at the break point via homology-directed repair (HDR).


The Cas9 from the bacteria Streptococcus pyogenes, abbreviated spCas9, is the most commonly used Cas9 variant. The fusion of Cas9 Nuclease NLS to GFP allows for visual confirmation of transfection as well as subsequent verification of Cas9 clearance from the cells. Cas9 Nuclease-GFP can also be used for FACS applications and screening. Cas9 Nuclease-GFP NLS contains a SV40 T antigen nuclear localization sequence (NLS) on the C-terminus of the protein. Our Cas9-GFP proteins have the Enhanced green fluorescent protein (eGFP).

abm offers a variety of Cas9 proteins. View our whole collection here.

 

Cas Type Nuclease
Cas Origin spCas9
Cas Protein Marker GFP
Concentration 10 µM, 1.88 mg/ml
Format General Enzyme supplied with 10X Reaction Buffer
Storage Buffer 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) Glycerol.
Storage Condition Store all components at -20°C.
Caution This product is distributed for laboratory research only. Not for diagnostic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. K148
Search CoA here
  • Majeau, N., et al. (2022). Serum extracellular vesicles for delivery of CRISPR-CAS9 ribonucleoproteins to modify the dystrophin gene. Molecular Therapy, 30(7), 2429–2442. https://doi.org/10.1016/j.ymthe.2022.05.023
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