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50 ml PBMC Separation Tube

Cat. No.

G7596

Unit

100 Tubes/Case

Price

$107.00

Cat. No.	G7596
Name	50 ml PBMC Separation Tube
Unit	100 Tubes/Case
Category	Culture Consumables
Description	PBMC Separation Tubes are pre-filled, sterile 50 mL tubes designed for the efficient isolation of peripheral blood mononuclear cells (PBMCs) from whole blood via density gradient centrifugation. Each tube is pre-layered with a density medium to streamline PBMC recovery while reducing handling time and variability. Integrated separation barrier prevents mixing of blood and gradient, eliminating manual layering Simple collection: MNCs easily harvested by pouring post-centrifugation High consistency reduces operator-dependent variability Fast protocol: Isolate PBMCs in as little as 15 minutes GMP-compliant manufacturing with third-party quality testing Triple sterile packaging with inner-layer lot tracking for full traceability Sterile (irradiated, SAL 10⁻⁶), DNase/RNase-free, non-pyrogenic, non-cytotoxic, and Mycoplasma-free Key Features: 50 mL volume per tube, pre-filled with density gradient medium Rapid, high-yield separation of PBMCs Sterile and ready-to-use Ideal for human or animal whole blood samples Ensures consistency and minimizes cross-contamination risk Undiluted volume: 4-17 ml Materials: Tube: Polypropylene (PP) Cap: High-density polyethylene (HDPE) Separation Bracket: MBS (Methyl methacrylate-butadiene-styrene) Compliance: Meets USP Class VI standards
Application	PBMC Isolation Protocol Prepare the Separation Medium Bring the separation medium to room temperature and keep it protected from light. Add Separation Medium Pipette 15 mL of the separation medium through the central hole of the built-in separation bracket. Ensure the medium level is above the bracket. Add Blood Sample Gently add the anticoagulated whole blood (diluted if necessary) down the side of the tube, avoiding direct entry through the central hole to prevent mixing with the separation medium. Centrifugation Centrifuge at 1200 ×g for 10 minutes at room temperature with the brake on. After centrifugation, the layers from top to bottom will be: plasma, PBMCs, separation medium, separation bracket, and erythrocytes/granulocytes. Collect PBMC Layer Carefully pour off the top layer containing PBMCs and plasma into a new clean centrifuge tube or pipette out the PBMC layer. Wash PBMCs Add phosphate-buffered saline (PBS) to the collected PBMCs and centrifuge at 250 ×g for 10 minutes at room temperature. Repeat this wash step once more. Resuspend Cells After the final wash, resuspend the PBMCs in PBS or an appropriate culture medium for downstream applications.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G7596

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Applied Biological Materials Inc. (abm) #1-3671 Viking Way, Richmond, BC, V6V 2J5, Canada
info@abmgood.com Local: 604-247-2416 Toll Free: 1-866-757-2414

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