Applied Cell Extracellular Matrix
| Cat. No. | G422 |
| Name | Applied Cell Extracellular Matrix |
| Description |
In cell biology, processes such as cell differentiation, cell cycle and cell survival are affected by signals stimulated through cell adhesion. Therefore adhesion plays a fundamental role in in vitro studies. ABM’s Applied Cell Extracellular Matrix composes mainly of type I collagen, isolated from rat (Rattus rattus) tail tendon. Type I collagen is the most abundant form of collagen and differs from other collagens by its low lysine hydroxylation and low carbohydrate composition. This product is suitable for usage as a solid gel or for coating of culture wares. ABM’s Applied Cell Extracellular matrix on culture surfaces have been shown to enhance cell attachment in a wide variety of cell lines such as hepatocytes, fibroblasts and epithelial cells. |
| Unit | 25ml |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G422 |
| How to coat flasks? | |
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To coat T-flasks, we recommend adding the desired amount of G422 to the flask and then putting the cap back on (if vented caps, tighten completely; for non-vented caps, keep them slightly loose). Once the incubation is complete, we recommend allowing the flasks to dry with the caps on to minimize the risk of introducing contamination.
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- Chesnokova, V. et al. "Growth hormone is a cellular senescence target in pituitary and nonpituitary cells" Proc Natl Acad Sci U S A 35:E3331-E3339 (2013). DOI: 10.1073/pnas.1310589110. PubMed: 23940366. Application: Cell Culture.
- Chesnokova, V; et al. "Growth hormone is a cellular senescence target in pituitary and nonpituitary cells" Proceedings of the National Academy of Sciences 35:E3331-E3339 (2013). PubMed: 23940366. Application: Cell culture.
- Sakaguchi, M et al. "miR-137 Regulates the Tumorigenicity of Colon Cancer Stem Cells through the Inhibition of DCLK1."" Molecular Cancer Research 14.4:354-362 (2016). DOI: 10.1158/1541-7786.MCR-15-0380. Application: Treat precultured plates.
- Robitaille, K et al. "High-throughput Functional Genomics Identifies Regulators of Primary Human Beta Cell Proliferation" Journal of Biological Chemistry 291.9:4614-4625 (2016). DOI: doi: 10.1074/jbc.M115.683912 . Application: Treat precultured plates.
- Chesnokova, V et al. "Growth hormone is permissive for neoplastic colon growth" Proceedings of the National Academy of Sciences of the United States of America 113.23:E3250–E3259 (2016). DOI: 10.1073/pnas.1600561113. Application: Cell culture.