BlasTaq™ HotStart DNA Polymerase

(Equivalent to Cat. No. G011, G039, G937.)

Eliminate non-specific amplification and primer-dimer formation!

BlasTaq™ HotStart DNA Polymerase is a strategically-engineered, next generation Taq Polymerase that has rapid extension rates, robust performance, and contains a proprietary antibody that blocks polymerase activity at low temperatures. HotStart allows for a convenient reaction set-up at room temperature without non-specific amplification and primer dimer formation. With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, while shortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase.

During the initial denaturation step, the antibody dissociates from the DNA polymerase and restores enzyme activity. This feature significantly reduces non-specific product formation that would otherwise compete for reagent availability offering higher specificity and improved yield of PCR products. BlasTaq™ has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. PCR products made with BlasTaq™ can be used with TA cloning vectors.

Product Features:

• No non-specific amplification or primer-dimers
• Improves yield of desired product
• Simplifies reaction setup
• Earn 2X the rewards points

ISO 13485:2016 MDSAP Certified Our PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).

 ¹ Buffer contains 1.5 mM Mg²⁺.

Note: This product is abm’s next generation of PCR enzymes and is functionally equivalent to Cat. No. G011 and G039 (HotStart DNA Polymerase) as well as Cat. No. G937 (SensTaq HotStart DNA Polymerase), with improved performance. Contact our customer service team [email protected] for more information.