BlasTaq™ HotStart DNA Polymerase

CAT.NOUNITPRICE
G595400 rxn
$295.00

Customize BlasTaq™ HotStart DNA Polymerase
1 x BlasTaq™ HotStart DNA Polymerase   + $0.00
2 x 5X BlasTaq™ Buffer   + $0.00

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BlasTaq™ HotStart DNA Polymerase
BlasTaq™ HotStart DNA Polymerase

In stock

$295.00

Summary
    Specifications


    Description

    (Equivalent to Cat. No. G011, G039, G937.)

    Eliminate non-specific amplification and primer-dimer formation!

    BlasTaq HotStart DNA Polymerase is a strategically-engineered, next generation Taq Polymerase that has rapid extension rates, robust performance, and contains a proprietary antibody that blocks polymerase activity at low temperatures. HotStart allows for a convenient reaction set-up at room temperature without non-specific amplification and primer dimer formation. With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, while shortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase.

    During the initial denaturation step, the antibody dissociates from the DNA polymerase and restores enzyme activity. This feature significantly reduces non-specific product formation that would otherwise compete for reagent availability offering higher specificity and improved yield of PCR products. BlasTaq has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. PCR products made with BlasTaq can be used with TA cloning vectors.

    Product Features:

    • No non-specific amplification or primer-dimers
    • Improves yield of desired product
    • Simplifies reaction setup
    • Earn 2X the rewards points

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    ISO 13485:2016 MDSAP Certified

    ISO 13485:2016 MDSAP Certified
    Our PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).

     

    Product ComponentQuantity
    BlasTaq HotStart DNA Polymerase 400 rxn (200 µl)
    5X BlasTaq Buffer 1 2 x 1.0 ml

     1 Buffer contains 1.5 mM Mg2+.

    Note: This product is abm’s next generation of PCR enzymes and is functionally equivalent to Cat. No. G011 and G039 (HotStart DNA Polymerase) as well as Cat. No. G937 (SensTaq HotStart DNA Polymerase), with improved performance. Contact our customer service team [email protected] for more information.


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    SKUG595
    Storage Condition

    Store at -20°C.

    Unit quantity400 rxn
    FAQs


    References


    15
    • Chung, G et al. "CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans" DNA Repair (Amst.) 10 (11):1174-1182 (2011). DOI: 10.1016/j.dnarep.2011.09.011. PubMed: 21968058. Application: PCR.