CRISPR Genomic Cleavage Detection Kit

Cat. No.
G932
Unit
25 Reactions
Price
$165.00
Cat. No. G932
Name CRISPR Genomic Cleavage Detection Kit
Unit 25 Reactions
Category Molecular Biology Enzymes and Kits
Description

abm’s CRISPR Genomic Cleavage Detection Kit is designed as a simple, rapid assay to verify your genomic editing process. Precision is a key quality in genome editing and gene modification methods. Confirmation of successful gene editing is an important step in any set up, and could save both time and resources if experimental insufficiencies are caught early on. 

CRISPR-edited samples are used as a template in PCR reactions targeting your specific region of interest. A pair of primers flanking each sgRNA target site is required to detect genomic cleavage using the kit, and this can be ordered from abm (Cat. No. C336 - sgRNA PCR Primer Pair Design & Synthesis Service). The products are then denatured and re-annealed to produce mismatches within the double strand. Our detection enzyme is able to recognize such mismatches and cleaves the strands to produce band sizes that are easily distinguishable upon gel analysis. The ready-to-use CRISPR Genomic Cleavage Detection Kit conveniently contains all the necessary reagents required, including a set of control template and primers to ensure reliable results. The control template has an approximate size of 750bp after PCR amplification and of 500bp and 250bp after the enzymatic cleavage. With a short processing time, this ready-to-use assay will be a great addition to any genomic-editing toolbox.

Kit Features:

  • Ease of use with simple steps
  • Rapid set-up
  • Streamlined protocol suitable for high-throughput applications
 
Product Component Quantity
Cell Lysis Buffer  1.25 ml
Protein Degarder 25 rxn (50 μl)
Detection Enzyme 13 μl
10X Detection Buffer 100 μl
Control Primer & Template 10 μl
MegaFi™ Pro Fidelity 2X MasterMix 1.25 ml
Nuclease-Free H2O 1.0 ml
Storage Condition

Store all components at -20°C.

Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G932
Search CoA here
There are no Documents for this product yet!
There are no FAQs for this product yet!
  • Chung, G et al. "CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans." DNA Repair (Amst.) 10 (11):1174- 1182 (2011). DOI: 10.1016/j.dnarep.2011.09.011. PubMed: 21968058. Application: PCR.
  • Chung, G et al. "CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans." DNA Repair (Amst.) 10 (11):1174- 1182 (2011). DOI: 10.1016/j.dnarep.2011.09.011. PubMed: 21968058. Application: PCR.
  • Ortiz-Cuaran, S. et al. (2022). Epithelial-to-mesenchymal transition promotes immune escape by inducing CD70 in non-small cell lung cancer. European Journal of Cancer, 169, 106-122.
This product has no review yet.
Controls and Related Product:

CAT.NO
E017
UNIT
100 U (100 μl)
PRICE
$52.50

CAT.NO
G467
UNIT
200 U (200 μl)
PRICE
$152.00

CAT.NO
E049
UNIT
500 U (50 μl)
PRICE
$310.00

CAT.NO
E086
UNIT
20 Reactions
PRICE
$150.00

CAT.NO
D518
UNIT
50 preparations
PRICE
$299.00

CAT.NO
D516
UNIT
100 preparations
PRICE
$175.00

CAT.NO
D514
UNIT
100 Preparations
PRICE
$110.00

CAT.NO
E081
UNIT
50 Reactions
PRICE
$195.00