Immortalized Human Prostate Stromal Cells (PM151T) - hTERT

Cat. No.
T0727
Unit
1x10⁶ cells / 1.0 ml
Price
$470.00

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Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing institution.

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Cat. No. T0727
Name Immortalized Human Prostate Stromal Cells (PM151T) - hTERT
Description

Prostate cancer research has been hindered by the fact that the cell types involved in the process of malignant transformation have proven difficult to develop into continuously growing cultures. Stromal smooth muscle cells established in primary culture after radical prostatectomy from a 62-year-old patient were subjected to retroviral infection with human telomerase catalytic subunit (hTERT), thus creating Immortalized Human Prostate Stromal Cells (PM151T) - hTERT. 

These cells were able to proliferate for at least 190 days, express genes associated with smooth muscle function (progesterone receptor, estrogen receptor-β, androgen receptor, smooth muscle-myosin heavy chain, calponin, SM22, smooth muscle α-actin and myocardin) and exhibit up-regulation for some of these markers as well as contractile behavior upon induction of differentiation. The generation of this cell line by way of a method that minimizes complex alterations results in a faithful in vitro model for the prostate, offering a tool for the study of not only normal development and physiology, but also the steps leading to malignancy. Characterization by Western blot, qRT-PCR, and transforming growth factor-β/endothelin A treatment.

Organism Human (H. sapiens)
Tissue Prostate
Donor History Male, 62
Growth Properties

Adherent, fibroblast

Cell Type Immortalized Cells
Unit 1x10⁶ cells / 1.0 ml
Storage Condition Vapor phase of liquid nitrogen, or below -130°C.
Shipping Conditions

Ship with dry ice.

Product Format Frozen
Intended Use This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
BioSafety II
Certificate of Analysis For batch-specific test results, refer to the applicable certificate of analysis that can be found at www.abmgood.com.
Growth Conditions Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. MCDB 131 medium (Sigma) + 15% decalcified horse serum (Sigma) + 10 mM pH 7.2 HEPES + 1X MEM Non-essential Amino Acid Solution + 5 µg/ml insulin (TM053) + 10 µg/ml transferrin (Sigma) + 10 nM estradiol + 100 nM dexamethasone (Sigma) + 5 ng/ml sodium selenite (Sigma) + 1 ng/ml basic FGF (Z101456) + 0.1 ng/ml EGF (Z100139) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂
Unpacking and Storage Instructions

1. Visually examine the packaging containers for signs of leakage or breakage.

2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130°C, preferably in liquid nitrogen vapor phase storage, until ready for use.

To ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130°C or in liquid nitrogen vapor phase. Do not store at -70°C, as it will result in loss of viability.

 

Thawing Protocol

1. Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.

2. Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.

3. Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes.

4. Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.

5. Incubate the cells at the recommended conditions.

Subculture Protocol

Volumes given below are for a T75 flask; proportionally increase or decrease the volume as required per culture vessel size. Subculture cells once the culture vessel is 80% confluent.

1. Aspirate the culture media, and add 2-3ml of pre-warmed 0.25% Trypsin-EDTA to the culture vessel.

2. Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in 37°C, for several minutes to facilitate detachment.

3. Neutralize Trypsin-EDTA by adding an equal volume of the complete growth media into the culture vessel.

4. Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 125xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.

5. Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired.

6. Incubate the cells at the recommended conditions.

Cryopreservation

Cryopreservation Medium (TM024), or complete growth media with 10% DMSO.

Seeding Density (cells/cm2) 2,500
Population Doubling Time (h) 20 - 30
Immortalization Method Immortalized with retrovirus containing hTERT
Expression

Progesterone receptor, estrogen receptor-β, androgen receptor, smooth muscle-myosin heavy chain, calponin, SM22, smooth muscle α-actin, myocardin, puromycin-resistance

Warranty abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period”.
Disclaimer

1. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each cell line. If you have any questions regarding this, please contact us at licensing@abmgood.com.

2. All test parameters provided in the CoA are conducted using abm's standardized culture system and The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

3. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of order placement. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location.

4. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic or any other non-RUO application(s).

5. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature are provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

6. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period".

Depositor Yeda Research and Development Co. Ltd.
Application

Research Use Only.

Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. T0727
Print & Download Datasheet
  • Ira Kogan, Naomi Goldfinger, Michael Milyavsky, Merav Cohen, Igor Shats, Gabriele Dobler, Helmut Klocker, Bohdan Wasylyk, Maureen Voller, Tilly Aalders, Jack A. Schalken, Moshe Oren, & Varda Rotter. (2006). hTERT-Immortalized Prostate Epithelial and Stromal-Derived Cells: an Authentic In vitro Model for Differentiation and Carcinogenesis. Cancer Research, 66:7, pg 3531-3540; DOI: 10.1158/0008-5472.CAN-05-2183


    Kogan, I., Goldfinger, N., Milyavsky, M., Cohen, M., Shats, I., Dobler, G., Klocker, H., Wasylyk, B., Voller, M., Aalders, T., Schalken, J. A., Oren, M., & Rotter, V. (2006). hTERT-immortalized prostate epithelial and stromal-derived cells: an authentic in vitro model for differentiation and carcinogenesis. Cancer research, 66(7), 3531–3540. https://doi.org/10.1158/0008-5472.CAN-05-2183

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