Immortalized Human Prostate Stromal Cells (PM151T) - hTERT

Prostate cancer research has been hindered by the fact that the cell types involved in the process of malignant transformation have proven difficult to develop into continuously growing cultures. Stromal smooth muscle cells established in primary culture after radical prostatectomy from a 62-year-old patient were subjected to retroviral infection with human telomerase catalytic subunit (hTERT), thus creating Immortalized Human Prostate Stromal Cells (PM151T) - hTERT. These cells were able to proliferate for at least 190 days, express genes associated with smooth muscle function (progesterone receptor, estrogen receptor-β, androgen receptor, smooth muscle-myosin heavy chain, calponin, SM22, smooth muscle α-actin and myocardin) and exhibit up-regulation for some of these markers as well as contractile behavior upon induction of differentiation. The generation of this cell line by way of a method that minimizes complex alterations results in a faithful in vitro model for the prostate, offering a tool for the study of not only normal development and physiology, but also the steps leading to malignancy.

For Research Use Only

Progesterone receptor, estrogen receptor-β, androgen receptor, smooth muscle-myosin heavy chain, calponin, SM22, smooth muscle α-actin, myocardin

Use of PriCoat^TM  T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.

The base medium for this cell line is MCDB 131 medium (Sigma). To make the complete growth medium, add the following components to the base medium: decalcified horse serum (Sigma) to a final concentration of 15%, pH 7.2 HEPES to a final concentration of 10 mM, MEM Non-essential Amino Acid Solution to a final concentration of 1X, insulin (Sigma) to a final concentration of 5 µg/mL, transferrin (Sigma) to a final concentration of 10 µg/mL, estradiol to a final concentration of 10 nM, dexamethasone (Sigma) to a final concentration of 100 nM, sodium selenite (Sigma) to a final concentration of 5 ng/mL, basic FGF (Sigma) to a final concentration of 1 ng/mL and EGF (Sigma) to a final concentration of 0.1 ng/mL.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
* Do not use heat-inactivated serum for cell culture unless specified otherwise.

1) Western blot; 2) qRT-PCR; 3) Transforming growth factor-β/endothelin A treatment

1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.

2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.

6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."