KtyI Stable Expressing Mouse Keratinocyte Cell Line

CAT.NOUNITPRICE
T62941x106 cells / 1.0 ml
$0.00

Specifications


Description

KtyI Stable Expressing Mouse Keratinocyte Cell Line are spontaneously immortalized cells. The cells express keratins (ktyl) and may be used in conjunction with Ktly Stable Knockout Mouse Keratinocyte Cell Line (T6293) to study the affects of the presence and absence of keratins.

SKUT6294
SpeciesMouse (M. musculus)
Tissue/Organ/Organ SystemSkin
Growth PropertiesAdherent
Cell MorphologyPolygonal
Seeding Density30,000 cells/cm2
Applications

For Research Use Only

Unit quantity1x106 cells / 1.0 ml
Cell TypeDrug Discovery Cell Lines
Expression Profile

Ktyl (Keratin)

Propagation Requirements

Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.

The base medium for this cell line is DMEM/Ham’s F12, low Calcium (50µM) (Biochrom). To make the complete growth medium, add the following components to the base medium: chelexed fetal calf serum (PAA, A15-151) to a final concentration of 10%, 2mM Glutamax (Invitrogen, 35050-038), 1X Pyruvate (PAA, S11-003), 0.18mM Adenin (Sigma, A8626-5G), 10 ng/ml EGF (Invitrogen 53003-018), 2.5 µg/ml insulin (Sigma-Aldrich, I-9278), 0.5 µl Hydroxycortison (Sigma-Aldrich, H4001-1G), 10-10 M Cholera toxin (Sigma,C-8052), and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Change media every 2-3 days
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
* Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Subculture Protocol

1. Aspirate media and wash cells with PBS (Ca2+ and Mg2+-free).
2. Add 2 ml of trypsin solution per 6 cm dish (3 ml for 10 cm dish) (0.025% trypsin/0.02% EDTA in PBS).
3. Incubate cells for 7-8 min at 37°C until cells detach.
4. Add same amount of fresh media and pipette the cells up and down several times to neutralize the trypsin/EDTA.
5. Pellet the cells by low speed centrifugation (1000 rpm for 4 minutes, RT), aspirate media, resuspend cells in 10 ml of fresh media and seed cells in G422-coated plates at 30,000 cells/cm2.

Preservation Protocol1. Freeze Medium: Complete growth medium with 20% chelexed-FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
Disclaimer

1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.

2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.

6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."

DepositorLeipzig University
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