pLenti-PGK-GFP Vector
-
Product Name
pLenti-PGK-GFP Vector -
Unit
1.0 µg -
Description
Empty versions of our most popular vectors to use as a control during your lentiviral experiments.
-
Promoter
PGK, SV40 -
Disclaimer
-
Caution
Not for diagnostic use.
Print/Download Datasheet
Search CoA here
Supporting Protocol
Alvarado, et al. "Coordination of self-renewal in glioblastoma by integration of adhesion and microRNA signaling" Neuro-Oncology 18.5:656 (2016). DOI: 10.1093/neuonc/nov196. Application: Lentiviral siRNA expression.
Hua, G et al. "The four and a half LIM domains 2 (FHL2) regulates ovarian granulosa cell tumor progression via controlling AKT1 transcription" Cell Death Dis. 7:e2297 (2016). DOI: 10.1038/cddis.2016.207.
Yao, Y et al. "Laminin regulates PDGFRβ+ cell stemness and muscle development" Nat. Commun. 7: (2016). DOI: 10.1038/ncomms11415. Application: Gene overexpression.
Kim, et al. "Characterization of the crosslinking kinetics of multi-arm poly(ethylene glycol) hydrogels formed via Michael-type addition" Soft Matter 12.7:2076 (2016). DOI: 10.1039/c5sm02668g. Application: Transfection.
Kong, J et al. "Long non-coding RNA LINC01133 inhibits epithelial–mesenchymal transition and metastasis in colorectal cancer by interacting with SRSF6" Cancer Letters 280.2:476-484 (2016). DOI: 10.1016/j.canlet.2016.07.015. Application: Overexpression.
Hotta, R et al. "Isogenic enteric neural progenitor cells can replace missing neurons and glia in mice with Hirschsprung disease" Neurogastroenterology & Motility 28.4:498–512 (2015). DOI: 10.1111/nmo.12744. Application: Expression.
Deng, et al. "An efficient polyethylene glycol-mediated transformation system of lentiviral vector in Shiraia bambusicola" Process Biochem. 51.10:1357 (2016). DOI: 10.1016/j.procbio.2016.07.013. Application: Vector Construction.
Pandya, et al. "Transglutaminase 2 overexpression induces depressive-like behavior and impaired TrkB signaling in mice" Mol. Psychiatry 2016: (2016). DOI: 10.1038/mp.2016.145. Application: Lentivirus Production.
Zhang, et al. "The construction and proliferative effects of a lentiviral vector capable of stably overexpressing SPINK1 gene in human pancreatic cancer AsPC-1 cell line" Tumour Biology 37.5:5847 (2016). DOI: 10.1007/s13277-015-4405-z. Application: Generation of lentiviral vectors.
Bhatt, S et al. "OCT-4: a novel estrogen receptor-α collaborator that promotes tamoxifen resistance in breast cancer cells" Oncogene 35.1:5722-5734 (2016). PubMed: 27065334. Application: Transfection.
Wang, et al. "Interleukin 6 induces expression of NADPH oxidase 2 in human aortic endothelial cells via long noncoding RNA MALAT1" Die Pharmazie - An International Journal of Pharmaceutical Sciences 71.10:592 (2016). DOI: 10.1691/ph.2016.6598. Application: Transduce HAOECs.
ABM community
Verified customer
Asked on Jan 30 2026
Answer
abm lentiviral transfer vectors use the third generation lentivirus system. It is based on the inactivated HIV genome. Note that our lentivirus packaging plasmids cannot be integrated into the host and are transiently expressed.
ABM Scientific Support
Answered on Jan 30 2026
ABM community
Verified customer
Asked on Mar 24 2025
Answer
We recommend using abm’s 2nd Generation Packaging System Mix (Cat. No. LV003) or 3rd Generation Packaging System Mix (Cat. No. LV053). abm’s lentiviral vectors have been tested and are compatible with Invitrogen’s packaging mix; we have not tested other suppliers and cannot guarantee compatibility.
ABM Scientific Support
Answered on Mar 24 2025
ABM community
Verified customer
Asked on Mar 24 2025
Answer
Higher MOI will provide more copies of the antibiotic resistance gene per cell. Cells containing multiple copies of the resistance gene can withstand higher antibiotic concentrations compared to those at lower MOIs. The concentration of antibiotic should be adjusted to a level that will cause selection for the desired population of transduced cells without going below the minimum antibiotic concentration you have established in your killing curve.
ABM Scientific Support
Answered on Mar 24 2025
ABM community
Verified customer
Asked on Jan 30 2026
Answer
MOI (Multiplicity of Infection) refers to the number of viral particles per cell used in the infection, e.g. an MOI of 5 indicates that there are five infectious units (IU) or transducing units (TU) for every cell. MOI is determined by calculating the numbers of viral particles added per well then divide this number by the number of cells seeded into the well. We also recommend transducing the cells with a range of MOIs as different cell types may require different MOIs for successful transduction.
MOI = Product Titer (IU/ml) x Virus Volume (ml) / Total Cell Number
MOI = Product Titer (IU/ml) x Virus Volume (ml) / Total Cell Number
ABM Scientific Support
Answered on Jan 30 2026
ABM community
Verified customer
Asked on Jan 30 2026
Answer
The concentration must be optimized for each cell type. Typical selection amounts are around 0.1 - 0.5µg per ml.
ABM Scientific Support
Answered on Jan 30 2026
ABM community
Verified customer
Asked on Mar 24 2025
Answer
The standard RFP used in most of our vectors is mkate2. It has an excitation wavelength of 588nm and emission wavelength of 633nm.
ABM Scientific Support
Answered on Mar 24 2025
ABM community
Verified customer
Asked on Jan 30 2026
Answer
These are medium-high copy plasmids and should be propagated in a cloning E. coli strain such as DH5α. Typical yields from a 250ml culture is 300-500µg plasmid DNA.
ABM Scientific Support
Answered on Jan 30 2026
ABM community
Verified customer
Asked on Jan 01 1970
Answer
There are no in frame start and stop codons after the EF1a promoter in this vector. This is a blank control vector and it is designed to not express anything from the EF1a promoter.
ABM Scientific Support
Answered on Jan 01 1970
ABM community
Verified customer
Asked on Jan 30 2026
Answer
MOI stands for multiplicity of infection. Theoretically, an MOI of 1 will provide 1 virus particle for each cell on a plate, while an MOI of 10 represents ten virus particles per cell. However, several factors can influence the optimal MOI including cell line, cell type, transduction efficiency and gene of interest. We recommend first establishing an optimal MOI for each cell line. This can be done using a range of MOIs (0, 0.5, 1, 2, 5, 10, 50) to determine the MOI required to obtain optimal gene expression
ABM Scientific Support
Answered on Jan 30 2026
There are currently no reviews for LV628.
Please use the link above to contact us or submit feedback about this product.
×
Current vector selected:
pLenti-PGK-GFP Vector
Cat. No.
LV628
Plasmid DNA Services
Midiprep Plasmid Amplification
10-20 µg
$75.00
Maxiprep Plasmid Amplification
80-100 µg
$150.00
Bacterial Agar Stab
1 stab
$40.00
Controls and Related Products
DNAfectin™ Plus Transfection Reagent
G2500
1.0 ml
$245.00
Susfectin™ Transfection Reagent
G4000
1.0 ml
$245.00
AAViralEntry™ Transduction Enhancer (100X)
G516
1.0 ml
$190.00
AAV-CMV-Blank Control Vector
AAV001a
1 μg
$99.00
Empty
×
Current vector selected:
pLenti-PGK-GFP Vector
Cat. No.
LV628
Controls and Related Products
DNAfectin™ Plus Transfection Reagent
G2500
1.0 ml
$245.00
Susfectin™ Transfection Reagent
G4000
1.0 ml
$245.00
AAViralEntry™ Transduction Enhancer (100X)
G516
1.0 ml
$190.00
AAV-CMV-Blank Control Vector
AAV001a
1 μg
$99.00
Empty
×
The vector and the related service will be deleted together.
×
Add the Blank Control Vector to cart?