Scrambled siRNA Lentivirus

CAT.NOUNITPRICE
LVP0152x200µl
$285.00

Specifications


DescriptionThis ready-to-use lentivirus is part of abm’s iLenti™ RNAi Expression System and can be used to knockdown your gene of interest in tranduced target cells. A GFP reporter and puromycin selection marker allow for selection of successfully transduced target cells.
SKULVP015
Unit quantity2x200µl
Titer>107 IU/ml
AppearanceLiquid
FormatVirus
Product FeaturessiRNA Set of 4 Targets (Pooled)
SystemLentivirus
Selection MarkerPuromycin
Sequencing PrimersTACGTCCAAGGTCGGGCAGGAAGA
Vector MappiLenti-siRNA
Shelf Life1 year when at -80°C
Storage ConditionLentiviruses are shipped with dry ice. For long term storage, it is recommended to store the viruses at -80°C in small aliquots to avoid repeated freeze-thaw cycles.
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References


10
  • Koo, JW et al. "Epigenetic basis of opiate suppression of Bdnf gene expression in the ventral tegmental area" Nat. Neurosci. 8(3):415-422 (2015). DOI: 10.1038/nn.3932. PubMed: 25643298.
  • Ramachandra, CJ et al. "ErbB4 Activated p38γ MAPK Isoform Mediates Early Cardiogenesis Through NKx2.5 in Human Pluripotent Stem Cells" Stem Cells : (2015). DOI: 10.1002/stem.2223. PubMed: 26418945.
  • Korb, E et al. "BET protein Brd4 activates transcription in neurons and BET inhibitor Jq1 blocks memory in mice" Nat Neurosci 18(10):1464-1473 (2015). DOI: 10.1038/nn.4095. PubMed: 26301327.
  • Ramachandra, C et al. "ErbB4 Activated p38γ MAPK Isoform Mediates Early Cardiogenesis Through NKx2.5 in Human Pluripotent Stem Cells" Stem Cells 34.2:288 (2016). DOI: 10.1002/stem.2223. Application: RNA Interference.
  • Shimizu, T et al. "Microglia-Induced Activation of Noncanonical Wnt Signaling Aggravates Neurodegeneration in Demyelinating Disorders" Mol. Cell. Biol. 36.21:2728-2741 (2016). DOI: 10.1128/MCB.00139-16. Application: Research.
  • Wang, et al. "Interleukin 6 induces expression of NADPH oxidase 2 in human aortic endothelial cells via long noncoding RNA MALAT1" Die Pharmazie - An International Journal of Pharmaceutical Sciences 71.10:592 (2016). DOI: 10.1691/ph.2016.6598. Application: Transduce HAOECs.
  • Li, W. Y., Zhang, W. T., Cheng, Y. X., Liu, Y. C., Zhai, F. G., Sun, P., ... & Wang, Y. "Inhibition of KLF7-targeting microRNA 146b promotes sciatic nerve regeneration"  Neuroscience bulletin  34(3):419-437 (2018).
  • Wei, X., Li, X., Yan, W., Zhang, X., Sun, Y., & Zhang, F. "SKP2 promotes hepatocellular carcinoma progression through nuclear AMPK-SKP2-CARM1 signaling transcriptionally regulating nutrient-deprived autophagy induction"  Cellular Physiology and Biochemistry  47(6):2484-2497 (2018).
  • Yu, S., Ren, H., Li, Y., Liang, X., Ning, Q., Chen, X., ... & Hu, T. "HOXA4-Dependent Transcriptional Activation of AXL Promotes Cisplatin-Resistance in Lung Adenocarcinoma Cells"  Anti-Cancer Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry-Anti-Cancer Agents)  18(14):2062-2067 (2018).
  • Zhang, M., Wang, B., Urabe, G., Huang, Y., Plutzky, J., Kent, K. C., & Guo, L.-W. "The BD2 domain of BRD4 is a determinant in EndoMT and vein graft neointima formation" Cellular Signalling 61:20–29 (2019). DOI: 10.1016/j.cellsig.2019.05.005.