Stable FUT8 Over-expressing HEK293S GnT I-/- Cell Line

T60101x106 cells / 1.0 ml


DescriptionHuman HEK293S GnT I-/- parental cell line was infected with recombinant lentivirus carrying the FUT8 gene in the pLV-CT vector to establish the Stable FUT8 Over-expressing HEK293S GnT I-/- Cell Line. FUT8 gene encodes for the fucosyltransferase 8 enzyme (α1,6-fucosyltransferase) which is involved with the transfer of fucose from GDP-fucose to N-linked type complex glycopeptides. It has been linked that FUT8 can independently fucosylate high mannose N-glycans without GnTI. Manipulated to over express FUT8, these cells can be utilized for studies relating to fucosylation and the involvement of the enzyme.
SpeciesHuman (H. sapiens)
Species descriptionHomo sapiens
Tissue/Organ/Organ SystemRenal
Growth PropertiesSuspension
Cell MorphologyRound
Seeding Density3x105 cells/ml
ApplicationsFor Research Use Only
Unit quantity1x106 cells / 1.0 ml
Pharmaceutical TargetEnzymes
CautionFor Research Use Only
Cell TypeDrug Discovery Cells
Propagation RequirementsThe base medium for this cell line is 50:50 mix of EX-Cell (Sigma) and FreeStyleTM 293 Medium (Life Technologies). Add 1 μg/ml puromycin (G264.) for selection. Grow cells in shaker shaking at 140 rpm/min.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.

Do not let cells grow above 3x106 cells/ml, and recommend to passage cells every 2-3 days.
QCPuromycin resistance determined (1 µg/ml puromycin)

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2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

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DepositorUniversity of Maryland

Supporting Protocol




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      • Yang, Q et al. " Home Current Issue Papers in Press Papers of the Week Reflections Minireviews Institution: SIMON FRASER UNIV Mammalian α-1,6-Fucosyltransferase (FUT8) Is the Sole Enzyme Responsible for the N-Acetylglucosaminyltransferase I-independent Core Fucosylation of High-mannose N-Glycans*" J Biol Chem. 291(21):11064-11071 (2016). PubMed: 27008861.