Stable FUT8 Over-expressing HEK293S GnT I-/- Cell Line
|T6010||1x106 cells / 1.0 ml|
|Description||Human HEK293S GnT I-/- parental cell line was infected with recombinant lentivirus carrying the FUT8 gene in the pLV-CT vector to establish the Stable FUT8 Over-expressing HEK293S GnT I-/- Cell Line. FUT8 gene encodes for the fucosyltransferase 8 enzyme (α1,6-fucosyltransferase) which is involved with the transfer of fucose from GDP-fucose to N-linked type complex glycopeptides. It has been linked that FUT8 can independently fucosylate high mannose N-glycans without GnTI. Manipulated to over express FUT8, these cells can be utilized for studies relating to fucosylation and the involvement of the enzyme.|
|Species||Human (H. sapiens)|
|Species description||Homo sapiens|
|Seeding Density||3x105 cells/ml|
|Applications||For Research Use Only|
|Unit quantity||1x106 cells / 1.0 ml|
|Caution||For Research Use Only|
|Cell Type||Drug Discovery Cells|
|Propagation Requirements||The base medium for this cell line is 50:50 mix of EX-Cell (Sigma) and FreeStyleTM 293 Medium (Life
Technologies). Add 1 μg/ml puromycin (G264.) for
selection. Grow cells in shaker shaking at 140 rpm/min. Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
Do not let cells grow above 3x106 cells/ml, and recommend to passage cells every 2-3 days.
|QC||Puromycin resistance determined (1 µg/ml puromycin)|
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3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.
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|Depositor||University of Maryland|
- Yang, Q et al. " Home Current Issue Papers in Press Papers of the Week Reflections Minireviews Institution: SIMON FRASER UNIV Mammalian α-1,6-Fucosyltransferase (FUT8) Is the Sole Enzyme Responsible for the N-Acetylglucosaminyltransferase I-independent Core Fucosylation of High-mannose N-Glycans*" J Biol Chem. 291(21):11064-11071 (2016). PubMed: 27008861.