Stable GS-3A4-HepG2 Cell Line
|T6016||1x106 cells / 1.0 ml|
|Description||The parental cell line of GS-3A4-HepG2 (HepG2) was derived from a human hepatoma and was transformed with a vector containing the Glutamine Synthetase gene allowing for ammonia metabolism and the 3A4 gene for drug metabolism. This transformation allows for activity in the immortalized cell line that is similar to primary hepatocytes but with a greater ability to metabolise drugs (3A4 activity). This cell line is highly functional in long term culture and therefore ideal for continuous research into liver function and metabolism. Previous studies have explored the cell lines use into bioartificial liver support systems. These systems are new technologies being developed to treat cases of liver damage where function needs to be supported during liver regeneration. The GS-3A4-HepG2 cell line may be particularly useful to metabolism drugs during long term experiments.|
|Species||Human (H. sapiens)|
|Applications||For Research Use Only|
|Seeding Density||20,000 - 40,000 cells/cm2|
|Population Doubling Time||13 - 23 hours|
|Donor Age||15 years old|
|Cell Type||Drug Discovery Cell Lines|
Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.
The base medium for this cell line is Prigrow X medium available at abm; Cat. No. TM6016.
|Preservation Protocol||1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.|
2. Storage Temperature: Liquid nitrogen vapour phase.
|Unit quantity||1x106 cells / 1.0 ml|
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