Stable GS-3A4-HepG2 Cell Line

T60161x106 cells / 1.0 ml


DescriptionThe parental cell line of GS-3A4-HepG2 (HepG2) was derived from a human hepatoma and was transformed with a vector containing the Glutamine Synthetase gene allowing for ammonia metabolism and the 3A4 gene for drug metabolism. This transformation allows for activity in the immortalized cell line that is similar to primary hepatocytes but with a greater ability to metabolise drugs (3A4 activity). This cell line is highly functional in long term culture and therefore ideal for continuous research into liver function and metabolism. Previous studies have explored the cell lines use into bioartificial liver support systems. These systems are new technologies being developed to treat cases of liver damage where function needs to be supported during liver regeneration. The GS-3A4-HepG2 cell line may be particularly useful to metabolism drugs during long term experiments.
SpeciesHuman (H. sapiens)
Tissue/Organ/Organ SystemLiver
Cell MorphologyEpithelial-like
ApplicationsFor Research Use Only
Seeding Density20,000 - 40,000 cells/cm2
Population Doubling Time13 - 23 hours
Donor GenderMale
Donor GenderMale
Donor Age15 years old
Donor EthnicityCaucasian
Cell TypeDrug Discovery Cell Lines
Growth PropertiesAdherent
Propagation Requirements
Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.

The base medium for this cell line is Prigrow X medium available at abm; Cat. No. TM6016.
Change media every 2-3 days.
Carbon dioxide (CO2): 5%; Temperature: 37.0C.

Preservation Protocol1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
Unit quantity1x106 cells / 1.0 ml

Supporting Protocol




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