Stable Human ER Positive Breast Epithelial Cell Line

CAT.NOUNITPRICE
T60211x106 cells / 1.0 ml
$0.00

Specifications


Description

Stable Human ER Positive Breast Cells expresses endogenous estrogen receptors (ER) in culture. It is a viable cell culture system enabling end users to conduct studies with oestrogen and screening of drug candidates suitable for breast cancer. The cells are a reliable source of renewable culture as the cells is immortalized with hTERT, thus limits batch to batch variation and gives expression of the estrogen receptors to manipulate.

SKUT6021
SpeciesHuman (H. sapiens)
Tissue/Organ/Organ SystemBreast
Growth PropertiesAdherent
Cell MorphologyEpithelial-Like
Seeding Density10,000 - 20,000 cells/cm2
Applications

For Research Use Only

Mammalian Selection MarkerNeomycin
Unit quantity1x106 cells / 1.0 ml
Pharmaceutical TargetNH Receptors
Induction Method

Infected with replication-incompetent lentivirus carrying the pBABE-neo-hTERT vector

Cell TypeDrug Discovery Cells
Propagation Requirements
Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.
The base medium for this cell line is Prigrow IV medium available at abm, Cat. No. TM004. To make the complete growth medium, add the following components to the base medium: 0.5??g/ml hydrocortisone, 5??g/ml insulin, 10?ng/ml cholera toxin (Sigma), 10?ng/ml epidermal growth factor (Peprotech), 1.8 × 10–4?M adenine, 10??M Y-27632 (Y0503, Sigma), fetal bovine serum (TM999) to a final concentration of 5%, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
QC

1) FACS used to isolate ER-positive expressing breast cells; 2) Immunpcytochemical staining used to detect the presence of ER

Disclaimer

1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.

2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.

6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."

Documents


Supporting Protocol
MSDS

    QC

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      References


      1
      • AJ, Fridriksdottir et al. "Propagation of oestrogen receptor-positive and oestrogen-responsive normal human breast cells in culture" Nat Commun. 6:8786 (2015). DOI: 10.1038/ncomms9786.