Stable Human ER Positive Breast Epithelial Cell Line
|T6021||1x106 cells / 1.0 ml|
|Description||Stable Human ER Positive Breast Cells expresses endogenous estrogen receptors (ER) in culture. It is a viable cell culture system enabling end users to conduct studies with oestrogen and screening of drug candidates suitable for breast cancer. The cells are a reliable source of renewable culture as the cells is immortalized with hTERT, thus limits batch to batch variation and gives expression of the estrogen receptors to manipulate.|
|Species||Human (H. sapiens)|
|Species description||Homo sapiens|
|Seeding Density||10,000 - 20,000 cells/cm2|
|Applications||For Research Use Only|
|Unit quantity||1x106 cells / 1.0 ml|
|Pharmaceutical Target||NH Receptors|
|Induction Method||Infected with replication-incompetent lentivirus carrying the pBABE-neo-hTERT vector|
|Caution||For Research Use Only|
|Cell Type||Drug Discovery Cells|
Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.The base medium for this cell line is Prigrow IV medium available at abm, Cat. No. TM004. To make the complete growth medium, add the following components to the base medium: 0.5??g/ml hydrocortisone, 5??g/ml insulin, 10?ng/ml cholera toxin (Sigma), 10?ng/ml epidermal growth factor (Peprotech), 1.8 × 10–4?M adenine, 10??M Y-27632 (Y0503, Sigma), fetal bovine serum (TM999) to a final concentration of 5%, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
|QC||1) FACS used to isolate ER-positive expressing breast cells; 2) Immunpcytochemical staining used to detect the presence of ER; 3) 500 μg/ml G418 used for selection|
1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.
|Depositor||University of Copenhagen|
- AJ, Fridriksdottir et al. "Propagation of oestrogen receptor-positive and oestrogen-responsive normal human breast cells in culture" Nat Commun. 6:8786 (2015). DOI: 10.1038/ncomms9786.