Taq DNA Polymerase
Cat. No.
G009
Unit
200 rxn
Price
$21.00
| Cat. No. | G009 | ||||||||||
| Name | Taq DNA Polymerase | ||||||||||
| Unit | 200 rxn | ||||||||||
| Category | PCR Polymerase | ||||||||||
| Description |
(Equivalent to Cat. No. G008 and G126.) Your cost-effective choice for routine amplification!Taq DNA Polymerase is a highly thermostable DNA Polymerase that catalyzes the 5’-3’ synthesis of DNA. This polymerase has 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. PCR products made with Taq can be used with TA cloning vectors. Product Features:
1 Buffer contains 1.5 mm Mg2+
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| Storage Condition |
Store at -20°C. |
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| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G009 |
Datasheet
Search CoA here
| Does the polymerase leaves a poly-A tail? | |
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It doesn't leave a poly-A tail but it does leave an "dA" overhang at the 3' ends.
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| I am observing positive results in my negative controls (no template added) when attempting to detect gene sequences in a bacterial strain. I suspect this may be due to residual bacterial DNA from the DNA polymerase. What steps should I take to address this issue? | |
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The presence of residual bacterial DNA is quite common in commercially available DNA polymerases, as most are expressed and purified using E. coli recombinant systems. In this case, we recommend switching to our Ultra-Pure BlasTaq™ 2X PCR MasterMix (Cat. No. G885). This product undergoes a rigorous multi-step purification protocol utilizing physical, chemical, and enzymatic methods to maximize the removal of contaminating genomic DNA.
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- KESKİN, Mahmut. "Microsatellite panels for parentage testing of Kilis goats reared in Turkey" Turkish journal of veterinary and animal science 43(1):94-101 (2019). DOI: 10.3906/vet-1809-21.
- Li, J., Liu, X.B., Zhao, Z.W., Yang, Z.L. "Genetic diversity, core collection and breeding history of Pleurotus ostreatus in China" Mycoscience 60(1):14-24 (2019). DOI: 10.1016/j.myc.2018.07.002.
- Liu, Q., Guo, X., Xun, G., Li, Z., Chong, Y., Yang, L., … Feng, Y. "A-Star, an Argonaute-directed System for Rare SNV Enrichment and Detection" : (2019). DOI: 10.1101/803841.
- Paltrinieri, S., Zambon, Y., Mori, N., Canel, A., Bertaccini, A., & Contaldo, N. "Phytoplasmas detected in insects and spontaneous vegetation near vineyards with yellows diseases in Italy" Phytopathogenic Mollicutes 9(1):55 (2019). DOI: 10.5958/2249-4677.2019.00028.8.
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