α3β2β4 nAChR Stable Expressing HEK293 Cell Line

CAT.NOUNITPRICE
T64201x106 cells / 1.0 ml
$0.00

Specifications


Description

It has been shown that neurons in constant exposure to nicotine will experience an up-regulation in nicotinic acetylcholine receptor (nAChR) expression. To generate this stable cell line nAChR was first transfected into the HEK293 cells then followed by the introduction of the rabbit polyclonal antibodies α3β2β4 via the pBUD vector. These stable cells were selected for in zeocin-containing complete media.

SKUT6420
SpeciesHuman (H. sapiens)
Tissue/Organ/Organ SystemKidney
Cell MorphologyEpithelial-like
Applications

For Research Use Only

Seeding Density30,000 - 50,000 cells/cm2
Population Doubling Time30 - 40 hours
Cell TypeDrug Discovery Cell Lines
Growth PropertiesAdherent
Expression Profile

pBudCE4.1 vector; zeocin resistance

Preservation ProtocolFreeze Medium: 90% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
Unit quantity1x106 cells / 1.0 ml
QC

1) Western blot; 2) Immunocytochemistry

Documents


Supporting Protocol
MSDS

    QC

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