α3β2 nAChR Stable Expressing HEK293 Cell Line
|T6419||1x106 cells / 1.0 ml|
It has been shown that neurons in constant exposure to nicotine will experience an up-regulation in nicotinic acetylcholine receptor (nAChR) expression. To generate this stable cell line nAChR was first transfected into the HEK293 cells then followed by the introduction of the rabbit polyclonal antibodies α3β2 via the pBUD vector. These stable cells were selected for in zeocin-containing complete media.
|Species||Human (H. sapiens)|
For Research Use Only
|Seeding Density||30,000 - 50,000 cells/cm2|
|Population Doubling Time||30 - 40 hours|
|Cell Type||Drug Discovery Cell Lines|
pBudCE4.1 vector; zeocin resistance
|Preservation Protocol||Freeze Medium: 90% FBS and 10% DMSO.|
2. Storage Temperature: Liquid nitrogen vapour phase.
|Unit quantity||1x106 cells / 1.0 ml|
1) Western blot; 2) Immunocytochemistry
There are no references for this product yet!