AaCas12b Lentiviral Vector
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Product Name
AaCas12b Lentiviral Vector -
Unit
10 µg -
Description
Lentiviral vector expressing the CRISPR-associated endonuclease AaCas12b derived from Alicyclobacillus acidiphilus. AaCas12b is part of the Class 2 Type VB CRISPR system, allowing it to target and cleave DNA via a sgRNA with the PAM sequence 5'-TTN-3.' It is noteable for its compact size and high specificity making it a promising new tool for CRISPR genome editing.
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Storage Condition
-20°C or below.
The main advantage of AaCas12b over SpCas9 is that AaCas12b is smaller, highly specific, and functions as a temperature-tolerant nuclease. Its compact size makes it easier to deliver with AAV vectors and its stringent PAM recognition reduces off-target activity compared to spCas9. Additionally, AaCas12b produces staggered DNA cuts rather than blunt ends, which can improve repair outcomes and broaden its utility for genome engineering and therapeutic applications.