Cas9 Proteins

CRISPR is the most versatile technology for genome editing and abm offers the largest selection of Cas9 proteins for CRISPR experiments. Cas9 Ribonucleoproteins (RNPs) are Cas9 proteins in complex with in vitro transcribed sgRNA. Cas9 RNPs can be directly tranfected into cells to achieve gene editing.

The Cas9 RNP delivery method has several advantages over other Cas9 delivery methods:

  • There is no transcription or translation required so editing is much more rapid and transient
  • Cas9 proteins are compatible with a wide range of organisms without consideration such as codon optimization or promoter compatibility
  • No plasmid or virus means no risk of Cas9 or sgRNA integration into the genome

Cas9 proteins and sgRNA can also be used for cutting DNA in vitro. Applications for this technique include pre-screening of sgRNA candidates on PCR amplified versions of the target gene of interest, or digestion of plasmids where no restriction site is available.

 

Our Cas9 protein collection includes:

TypeDescriptionKnowledge Base
Cas9 Nuclease High-efficiency CRISPR genome editing nuclease available as spCas9saCas9 (smaller than spCas9 and suitable for packaging into AAV expression systems) or Cpf1 (targets T-rich genomic sites). Read more about different Cas9 nucleases here.
Cas9 Nickase Generates single-stranded nicks at target site for repair via the Homology Directed Repair (HDR) pathway to minimize off-target cleavage effects. Read more about Cas9 nickases here.
Cas9 Double Mutant (null mutant, dCas9) Cas9 with non-functional cleavage domains but with gene targeting function via sgRNA still intact. Useful as a tool for reversible knockdown of gene expression. Read more about Cas9 null mutants here.

Other CRISPR Delivery Tools:

Looking for our CRISPR Cas9+sgRNA ready-to-use knockout vectors and viruses? Click here.

Looking for our Cas9-only vectors and viruses? Click here.

Need recommendations on which CRISPR components you need for your experiment? Try our CRISPR Experimental Design Tool.

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  1. Sku: K188
    Price: $350.00
    Unit: 380 μg (2.5 nmol/ 250µL)
    Using Cpf1 (a.k.a. Cas12a) in your CRISPR experiment offers several advantages over other CRISPR-associated nucleases. Due to the T-rich PAM sequence (TTTN), Cpf1 enables editing in regions unable to be targeted by Cas9. Cpf1 can be used with...


  2. Sku: K088
    Price: $70.00
    Unit: 38 μg (250 pmol/ 25µL)
    Using Cpf1 (a.k.a. Cas12a) in your CRISPR experiment offers several advantages over other CRISPR-associated nucleases. Due to the T-rich PAM sequence (TTTN), Cpf1 enables editing in regions unable to be targeted by Cas9. Cpf1 can be used with...


  3. Sku: K049
    Price: $50.00
    Unit: 9.5µg (50pmol/ 50µL)
    The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. One concern with the current CRISPR...


  4. Sku: K149
    Price: $145.00
    Unit: 47µg (250pmol/ 25µL)
    The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. One concern with the current CRISPR...


  5. Sku: K034
    Price: $45.00
    Unit: 8.0µg (50pmol/ 50µL)
    The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. One concern with the current CRISPR...


  6. Sku: K134
    Price: $125.00
    Unit: 40µg (250pmol/ 25µL)
    The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. One concern with the current CRISPR...


  7. Sku: K032
    Price: $45.00
    Unit: 8.0µg (50pmol/ 50µL)
    The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. One concern with the current CRISPR...


  8. Sku: K132
    Price: $125.00
    Unit: 40µg (250pmol/ 25µL)
    The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. One concern with the current CRISPR...


  9. Sku: K038
    Price: $45.00
    Unit: 8.0µg (50pmol/ 50µL)
    The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. One concern with the current CRISPR...


  10. Sku: K138
    Price: $125.00
    Unit: 40µg (250pmol/ 25µL)
    The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. One concern with the current CRISPR...

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