CRISPR is the most versatile technology for genome editing and abm offers the largest selection of Cas9 proteins for CRISPR experiments. Cas9 Ribonucleoproteins (RNPs) are Cas9 proteins in complex with in vitro transcribed sgRNA. Cas9 RNPs can be directly tranfected into cells to achieve gene editing.
The Cas9 RNP delivery method has several advantages over other Cas9 delivery methods:
- There is no transcription or translation required so editing is much more rapid and transient
- Cas9 proteins are compatible with a wide range of organisms without consideration such as codon optimization or promoter compatibility
- No plasmid or virus means no risk of Cas9 or sgRNA integration into the genome
Cas9 proteins and sgRNA can also be used for cutting DNA in vitro. Applications for this technique include pre-screening of sgRNA candidates on PCR amplified versions of the target gene of interest, or digestion of plasmids where no restriction site is available.
Our Cas9 protein collection includes:
|Cas9 Nuclease||High-efficiency CRISPR genome editing nuclease available as spCas9, saCas9 (smaller than spCas9 and suitable for packaging into AAV expression systems) or Cpf1 (targets T-rich genomic sites).||Read more about different Cas9 nucleases here.|
|Cas9 Nickase||Generates single-stranded nicks at target site for repair via the Homology Directed Repair (HDR) pathway to minimize off-target cleavage effects.||Read more about Cas9 nickases here.|
|Cas9 Double Mutant (null mutant, dCas9)||Cas9 with non-functional cleavage domains but with gene targeting function via sgRNA still intact. Useful as a tool for reversible knockdown of gene expression.||Read more about Cas9 null mutants here.|
- Cas9 Nuclease GFP NLS Protein
- Cas9 Nuclease NLS Protein (High Concentration)
- Cas9 Null Mutant Protein (High Concentration)