Cas9 Proteins

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CRISPR is the most versatile technology for genome editing and abm offers the largest selection of Cas9 proteins for CRISPR experiments. Cas9 RNPs (Cas9 proteins in complex with in vitro transcribed sgRNA) can be directly tranfected into cells to achieve gene editing.


This method has several advantages over other Cas9 delivery methods:

  • There is no transcription or translation required so editing is much more rapid and transient
  • Cas9 proteins are compatible with a wide range of organisms without consideration such as codon optimization or promoter compatibility
  • No plasmid or virus means no risk of Cas9 or sgRNA integration into the genome


Cas9 proteins and sgRNA can also be used for cutting DNA in vitro. Applications for this technique include pre-screening of sgRNA candidates on PCR amplified versions of the target gene of interest, or even cutting of plasmids where no restriction site is available.


To learn more about CRISPR please visit our CRISPR Knowledge Base.



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Cas9 Proteins
Product Cat No. Quantity Price
Cas9 Nuclease Protein K008 50 pmol (50 µl), 1000 nM, 160 µg/ml $25.00
Cas9 Nuclease NLS Protein K030 50 pmol (50 µl), 1000 nM, 160 µg/ml $25.00
Cas9 Nickase D10A Protein K032 50 pmol (50 µl), 1000 nM, 160 µg/ml $45.00
Cas9 Nickase D10A NLS Protein K034 50 pmol (50 µl), 1000 nM, 160 µg/ml $45.00
Cas9 Nickase H840A Protein K036 50 pmol (50 µl), 1000 nM, 160 µg/ml $45.00
Cas9 Nickase H840A NLS Protein K038 50 pmol (50 µl), 1000 nM, 160 µg/ml $45.00
Cas9 Null Mutant Protein K040 50 pmol (50 µl), 1000 nM, 160 µg/ml $45.00
Cas9 Null Mutant NLS Protein K042 50 pmol (50 µl), 1000 nM, 160 µg/ml $45.00
 
High Concentration Cas9 Proteins
Product Cat No. Quantity Price
Cas9 Nuclease Protein K108 250 pmol (25 µl), 10 µM, 1.60 mg/ml $75.00
Cas9 Nuclease NLS Protein K130 250 pmol (25 µl), 10 µM, 1.60 mg/ml $75.00
Cas9 Nickase D10A Protein K132 250 pmol (25 µl), 10 µM, 1.60 mg/ml $125.00
Cas9 Nickase D10A NLS Protein K134 250 pmol (25 µl), 10 µM, 1.60 mg/ml $125.00
Cas9 Nickase H840A Protein K136 250 pmol (25 µl), 10 µM, 1.60 mg/ml $125.00
Cas9 Nickase H840A NLS Protein K138 250 pmol (25 µl), 10 µM, 1.60 mg/ml $125.00
Cas9 Null Mutant Protein K140 250 pmol (25 µl), 10 µM, 1.60 mg/ml $125.00
Cas9 Null Mutant NLS Protein K142 250 pmol (25 µl), 10 µM, 1.60 mg/ml $125.00
 
Cas9 GFP Proteins
Product Cat No. Quantity Price
Cas9 Nuclease GFP NLS Protein K048 50 pmol (50 µl), 1000 nM, 160 µg/ml $50.00
Cas9 Nuclease GFP NLS Protein K148 250 pmol (25 µl), 10 µM, 1.60 mg/ml $145.00
Cas9 D10A Nickase GFP NLS Protein K049 50 pmol (50 µl), 1000 nM, 160 µg/ml $50.00
Cas9 D10A Nickase GFP NLS Protein K149 250 pmol (25 µl), 10 µM, 1.60 mg/ml $145.00
Cas9 Null Mutant GFP NLS Protein K086 50 pmol (50 µl), 1000 nM, 160 µg/ml $50.00
Cas9 Null Mutant GFP NLS Protein K186 250 pmol (25 µl), 1000 nM, 160 µg/ml $145.00
 
saCas9 Proteins
Product Cat No. Quantity Price
saCas9 Nuclease Protein K044 50 pmol (50 μl), 1000 nM, 130 µg/ml $50.00
saCas9 Nuclease NLS Protein K045 50 pmol (50 μl), 1000 nM, 130 µg/ml $50.00
saCas9 Null Mutant Protein K046 50 pmol (50 μl), 1000 nM, 130 µg/ml $50.00
saCas9 Null Mutant NLS Protein K047 50 pmol (50 μl), 1000 nM, 130 µg/ml $50.00
 
High Concentration saCas9 Proteins
Product Cat No. Quantity Price
saCas9 Nuclease Protein K144 250 pmol (25 μl), 10 µM, 1.30 mg/ml $145.00
saCas9 Nuclease NLS Protein K145 250 pmol (25 μl), 10 µM, 1.30 mg/ml $145.00
saCas9 Null Mutant Protein K146 250 pmol (25 μl), 10 µM, 1.30 mg/ml $145.00
saCas9 Null Mutant NLS Protein K147 250 pmol (25 μl), 10 µM, 1.30 mg/ml $145.00
 
sgRNA Synthesis Kit
Product Cat No. Quantity Price
GeneCraft-R Express CRISPR sgRNA Synthesis Kit (S. pyogenes) G948 25 Reactions $275.00
GeneCraft-R Classic CRISPR sgRNA Synthesis Kit (S. pyogenes) G952 25 Reactions $235.00
 
Other Cas9 Related Products
Product Cat No. Quantity Price
CRISPR Genomic Cleavage Detection Kit G932 25 Reactions $165.00
OneScribe T7 Transcription Kit E081 50 Reactions $185.00
GFP Purified Protein 000033P 50 μg $75.00
RFP Purified Protein 000035P 500 μg $225.00
Proteinfectin™ Transfection Reagent G288 250 μl $850.00
Anti-Cas9 Antibody Y300079 100 μl $195.00
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More about CRISPR
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system allows for highly specific genomic disruption and replacement. This simple and robust system requires co-expression of two distinct components: (1) an exonuclease, Cas9 and (2) a target-specific guide RNA (gRNA). Cas9 expression in the target cell or host is achieved by lentiviral vector transfection, lentivirus transduction, or adenovirus transduction. Cas9 will unwind the DNA duplex and cleave both strands upon recognition of the target sequence by the gRNA. The resulting double stranded break is repaired by the non-homologous end joining (NHEJ) pathway, disrupting the open reading frame of the targeted gene by causing an InDel frameshift and/or premature stop codon.


Homology Directed Repair (HDR) with Cas9 Nuclease
In addition to NHEJ, cells are able to perform DNA repair by Homology Directed Repair (HDR), which can be exploited to introduce nucleotide modifications to genomic DNA. A DNA repair template containing the desired sequence is normally transfected into the cell along with the gRNA/Cas9 and must have a high degree of homology to the sequence immediately upstream and downstream of the double stranded break.


Cas9 Nickase for Homologous Recombination and Specificity
The Cas9 nuclease has been modified into a "nickase" so that one of its catalytic domains is inactive, resulting in a single stranded nick instead of a double stranded break at the target site on the genomic DNA. This modification reduces off-target effects in the event that the guide RNA (gRNA) imperfectly binds to an undesired DNA site because not one, but two gRNAs located in close proximity (<20 nucleotides) on opposite strands of the genomic DNA are required in order to create the desired double stranded break. An undesired single stranded nick in the genomic DNA can be quickly repaired by the Homology Directed Repair (HDR) pathway using the other intact strand as the template.

The Cas9 nickase can also be used to create a single stranded nick in the genomic DNA for introducing nucleotide modifications by homologous recombination if the template DNA containing the desired modification is transfected into the cell along with the gRNA/Cas9.


  Guide RNA requirement InDels induced? HDR induced? Recommended Application
Cas9 Nuclease One gRNA for each target Yes, most efficient Yes InDel, HDR (when accuracy, i.e. off-target effects, is not a big concern)
Cas9 Nickase One gRNA for each target Barely detectable Yes, less efficient HDR without InDel generation
Cas9 Nickase Two gRNAs for each target Yes, very efficient Yes InDel, HDR (when accuracy, i.e. off-target effects, is a concern)