EBV Virus (Epstein-Barr Virus)
| Cat. No. | G229 |
| Name | EBV Virus (Epstein-Barr Virus) |
| Unit | 5 x 1.0 ml |
| Unpacking and Storage Instructions |
For long term storage, it is recommended to store the viruses at -80°C in small aliquots to avoid repeated freeze-thaw cycles. |
| Description |
This Epstein-Barr virus (EBV) is purified from cell culture and provided at >106 IU/ml titer. EBV is also referred to as Human herpesvirus 4, and is a gamma herpesvirus of the genus Lymphocryptovirus. This EBV strain is not engineered but is a lab-adapted variant containing a characteristic ~12 kb genomic deletion relative to wild-type EBV. This deletion removes a subset of genes (primarily associated with lytic replication), resulting in a virus that is somewhat “deficient” compared to wild-type, but still highly efficient at transforming B cells. |
| Application |
Cell immortalization of B lymphocytes and sometimes T lymphocytes. |
| Expression System Type | EBV (Epstein-Barr Virus) |
| Caution |
This product is distributed for laboratory research only. The product contains wild-type virus purified from cell culture, handle accordingly (BSL-2). |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G229 |
| What is the general procedure for immortalizing cells? | |
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See our Cell Immortalization Handbook for more details.
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| Can you recommend a specific reagent and protocol to immortalize my cell line? | |
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Successful cell immortalization is unpredictable and must be determined experimentally, as results vary with species, tissue origin, growth characteristics and whether or not specific cell functions need to be preserved.
Our in-house experiments using the SV40T and hTERT lentiviruses have achieved the highest immortalization success rates in a variety of different cell types. |
| Do immortalized cells retain all characteristics of the original primary cell line? | |
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Not always. Some phenotypic or genetic changes may occur during immortalization. We recommend using our hTERT immortalization reagents as this method preserves phenotype and karyotype as well as minimizes genomic instability.
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| Do I need a control when performing cell immortalization experiments? | |
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Yes. Always include control cells that are not transduced in order to compare growth rates and detect senescence bypass.
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| How do I confirm successful cell immortalization? | |
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Assess long-term growth beyond normal passage limits and verify expression of the immortalizing genes via PCR, qPCR or Western blot.
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| Where can I learn more about cell immortalization? | |
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Check out our Learning Resources and Youtube video for more information.
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| Will this virus immortalize my primary cells? | |
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This is highly dependent on the immortalization reagent and cell type. Check out our Cell Immortalization Reagents Compatibility Chart for more information.
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