ADRM1 Stable Knockout HCT116 (Clone B11) Cell Line

CAT.NOUNITPRICE
T61621x106 cells / 1.0 ml
$0.00

Specifications


DescriptionADRM1 or Adhesion regulating molecule 1, is a component of the proteosome and ubiquitin receptor that recruits the deubiquitinating enzyme, ubiquitin carboxyl-terminal hydrolase L5. Known to undergo O-linked glycosylation, the dysregulation of ADRM1 has been implicated in carcinogenesis making this protein a possible target for drug studies. ADRM1 stable knockout HCT116 (Clone B11) cell line was generated through CRISPR-Cas 9 directed knock exon 4 within the ADRM1 sequence. Exon 4 was replaced with a puromycin resistance cassette and pBluescriptII, allowing for the selection of successfully transformed cells via puromycin resistance.
SKUT6162
SpeciesHuman (H. sapiens)
Tissue/Organ/Organ SystemColon
Donor GenderMale
Donor AgeDonor Info Not Disclosed
Donor DiseaseColorectal carcinoma
Donor EthnicityNot Disclosed
Seeding Density30,000 - 50,000 cells/cm2
Population Doubling Time25 - 35 hours
ApplicationsFor Research Use Only
Unit quantity1x106 cells / 1.0 ml
Cell TypeDrug Discovery Cells
Expression ProfileADRM-1 knock out
Propagation RequirementsThe base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the completed growth medium, add the following components to the base medium: fetal calf serum to final concentration of 10%, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO2): 5%, Temperature: 33.0
Disclaimer

1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.

2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.

6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."

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