Atg5 Knockout Immortalized Mouse Kidney Epithelial Cell Line
|T3027||1x106 cells / 1.0 ml|
Autophagy is a catabolic process in which proteins and cellular organelles are degraded under metabolic stress through the lysosomal pathway. Essential autophagy-related genes (i.e. Atg 5 and Atg 7) play an important role in mammalian development, survival and maintaining metabolism and homeostasis. The Atg5 Knockout Immortalized Mouse Kidney Epithelial Cells (Atg5-/- iBMK) is derived from stable transformation of E1A and p53DD into primary kidney epithelial cells from mice deficient for Atg5. Together with the wild-type iBMK (T0082) and Atg7 Knockout iBMK (Atg7-/- iBMK, T3028), these cell lines are suitable in studying the relationships between autophagy and tumor suppression/cancer cell survival.
|Species||Mouse (M. musculus)|
|Species description||Mouse (C57BL/6J)|
|Seeding Density||30,000 - 50,000 cells/cm2|
|Population Doubling Time||43 - 53 hours|
For Research Use Only
|Unit quantity||1x106 cells / 1.0 ml|
|Caution||For Research Use Only|
|Cell Type||Drug Discovery Cells|
E-cadherin, CK8 and CK18
Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
1. Western blot was used to confirm the presence of E1A and p53DD gene in the immortalized cell line. 2. Western blot was used to check epithelial markers E-cadherin and cytokeratin expression. 3. PCR genotyping of the pup tail and western blot analysis were performed to verify the genotype of the cell line.
1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.
2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].
3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.
4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).
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7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
- Mathew, R et al. "Autophagy suppresses tumor progression by limiting chromosomal instability" Genes Dev. 21(11):1367-1381 (2007). DOI: 10.1101/gad.1545107. PubMed: PMC1877749.
- Mathew, R et al. "Autophagy suppresses tumorigenesis through elimination of p62" Cell 137(6):1062-1075 (2009). DOI: 10.1016/j.cell.2009.03.048. PubMed: PMC2802318.
- Mathew, R et al. "Immortalized mouse epithelial cell models to study the role of apoptosis in cancer" Methods in Enzymology 446:77-106 (2008). DOI: 10.1016/S0076-6879(08)01605-4.
- Mathew, R et al. "Assessing metabolic stress and autophagy status in epithelial tumors" Methods in Enzymology 453:53-81 (2009). DOI: 10.1016/S0076-6879(08)04004-4. PubMed: PMC2857509.
- Guo, JY et al. "Activated Ras requires autophagy to maintain oxidative metabolism and tumorigenesis" Genes Dev. 25(5):460-170 (2011). DOI: 10.1101/gad.2016311. PubMed: PMC3049287.
- Lau, A et al. "A noncanonical mechanism of Nrf2 activation by autophagy deficiency: direct interaction between keap1 and p62" Mol Cell Biol 30(13):3275-3285 (2010). DOI: 10.1128/MCB.00248-10. PubMed: PMC2897585.
- Kenific, C et al. "NBR1 enables autophagy-dependent focal adhesion turnover" The Journal of Cell Biology 212.5:577-590 (2016). DOI: 10.1083/jcb.201503075. Application: Cell culture.