B27 Supplement (50X), without Vitamin A
| Cat. No. | TM136 |
| Name | B27 Supplement (50X), without Vitamin A |
| Description |
B27 Supplement (50X), without Vitamin A is a serum-free additive formulated to support the growth, survival, and maintenance of neuronal and neural progenitor cells in vitro. It provides a defined mixture of vitamins, antioxidants, hormones, and other essential nutrients that enhance cell viability and reduce oxidative stress. This formulation is ideal for neurosphere cultures, primary neuronal cultures, stem cell–derived neural differentiation protocols, and a variety of organoid culture systems. By omitting vitamin A (retinyl acetate/retinol), it prevents retinoic acid–induced differentiation, helping neural stem cells and progenitor cells remain in an undifferentiated state. |
| Unit | 10 ml |
| Usage |
It is recommended to add B27 Supplement to a neuronal basal medium (e.g., Neuron Basal Medium - A) to achieve a final working concentration of 1X (2% v/v; 1:50 dilution). Alternative concentrations or media formulations may be used depending on experimental needs. |
| Sterility | Sterile |
| Appearance | Liquid |
| QC |
Each lot has been fully tested and optimized for its ability to support in vitro cell culture. The testing parameters include: adherence rate (where applicable), typical morphology, and metabolism of the tested cells. |
| Storage Condition | If unopened, keep frozen at−20°C, and protected from light. Once thawed, store at 2–8°C and use within 2 weeks. After addition to media, do not refreeze complete medium, as precipitation may occur. Store at 2–8°C in the dark and use within 2 weeks. |
| Caution |
For research use only. Not intended for therapeutic or diagnostic applications. |
| Disclaimer |
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| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. TM136 |