BAK Stable Knockdown HN12 Cell Line
|T6416||1x106 cells / 1.0 ml|
|Description||When cells undergo death triggered by chemotherapeutic agents the BCL-2-family-dependent mitochondrial apoptotic pathway is activated. By activating BAX and/or BAK one of the BCL-2 subgroups BH3-only proteins cause cytochrome to release from the mitochondria. shRNAs were introduced specific for BAK and BAX in HN12 cells so their roles can be studied. The results suggest that both BAX and BAK are required for apoptosis induced by simultaneous inhibition of BCL-2.|
|Species||Human (H. sapiens)|
|Applications||For Research Use Only|
|Population Doubling Time||24 - 36 hours|
|Donor Disease||Metastatic head and neck squamous cell carcinoma (HNSCC)|
|Cell Type||Drug Discovery Cell Lines|
|Expression Profile||Down-regulated BAK Vector information: shBAK: TRCN0000033466 TG GTACG AAGATTCTTC AAAT|
|Preservation Protocol||1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.|
2. Storage Temperature: Liquid nitrogen vapour phase.
|Unit quantity||1x106 cells / 1.0 ml|
|QC||1) Immunoprecipitation; 2) Western blot; 3) FACS; 4) Crystal violet assay; 5) Cell viability assays for Bliss Independence analysis|
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