BAK Stable Knockdown HN12 Cell Line

T64161x106 cells / 1.0 ml


DescriptionWhen cells undergo death triggered by chemotherapeutic agents the BCL-2-family-dependent mitochondrial apoptotic pathway is activated. By activating BAX and/or BAK one of the BCL-2 subgroups BH3-only proteins cause cytochrome to release from the mitochondria. shRNAs were introduced specific for BAK and BAX in HN12 cells so their roles can be studied. The results suggest that both BAX and BAK are required for apoptosis induced by simultaneous inhibition of BCL-2.
SpeciesHuman (H. sapiens)
Tissue/Organ/Organ SystemOther
Cell MorphologyEpithelial-like
ApplicationsFor Research Use Only
Population Doubling Time24 - 36 hours
Donor DiseaseMetastatic head and neck squamous cell carcinoma (HNSCC)
Cell TypeDrug Discovery Cell Lines
Growth PropertiesAdherent
Expression ProfileDown-regulated BAK Vector information: shBAK: TRCN0000033466 TG GTACG AAGATTCTTC AAAT
Preservation Protocol1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
Unit quantity1x106 cells / 1.0 ml
QC1) Immunoprecipitation; 2) Western blot; 3) FACS; 4) Crystal violet assay; 5) Cell viability assays for Bliss Independence analysis

Supporting Protocol




      There are no FAQs for this product yet!

      There are no references for this product yet!