Bax/Bak Double Knockout Immortalized Mouse Kidney Epithelial Cell Line

T30261x106 cells / 1.0 ml


DescriptionKey regulators of apoptosis, such as members of the BCL-2 family, have long been linked to the development of cancer. The Bax/Bak Double Knockout Immortalized Mouse Kidney Epithelial Cells (bax-/-/ bak-/- iBMK) is derived from stable transformation of E1A and p53DD into primary baby kidney epithelial cells from mice deficient for Bak and Bax. Bax and Bak are functionally redundant downstream regulators to the mechanism of apoptosis, therefore this double knockdown cell line provides a valuable insight into pro-apoptotic proteins and programmed cell death. Together with the wild-type iBMK (T0082), Bax knockout iBMK (bax-/- iBMK, T3024) and Bak Knockout iBMK (Bak-/- iBMK, T3025), these cell lines are suitable in studying the relationships between BCL-2 family and cancer progression, prognosis and significance to treatment.
SpeciesMouse (M. musculus)
Species descriptionMouse (C57BL/6J)
Tissue/Organ/Organ SystemRenal
Growth PropertiesAdherent
Cell MorphologyCuboidal
Seeding DensityThaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
ApplicationsFor Research Use Only
Unit quantity1x106 cells / 1.0 ml
Pharmaceutical TargetOther
CautionFor Research Use Only
Clone NameD3
Cell TypeDrug Discovery Cells
Expression ProfileE-cadherin, CK8 and CK18
Propagation Requirements
Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.
The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
QC1. Western blot was used to confirm the presence of E1A and p53DD gene in the immortalized cell line. 2. Western blot was used to check epithelial markers E-cadherin and cytokeratin expression. 3. PCR genotyping of the pup tail was performed to verify the genotype of the cell line.

1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.

2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.

6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."


Supporting Protocol




      There are no FAQs for this product yet!

      • Valero, JG et al. "µ-Calpain Conversion of Antiapoptotic Bfl-1 (BCL2A1) into a Prodeath Factor Reveals Two Distinct alpha-Helices Inducing Mitochondria-Mediated Apoptosis" PLoS One 7(6):e38620 (2012). PubMed: PMC3379997.
      • Degenhardt, K et al. "BAX and BAK mediate p53-independent suppression of tumorigenesis" Cancer Cell 2(3):193-203 (2002). PubMed: 11242152.
      • Zheng, JY et al. "Alterations in the characteristic size distributions of subcellular scatterers at the onset of apoptosis: effect of Bcl-xL and Bax/Bak" J. Biomed. Opt. 15(4):045002 (2010). DOI: 10.1117/1.3462933 20799797.
      • Leshchiner, ES et al. "Direct activation of full-length proapoptotic BAK" Proc Natl Acad Sci U S A. 110(11):E986–E995 (2013). DOI: 10.1073/pnas.1214313110. PubMed: PMC3600461.
      • Simmons, MJ et al. "Bfl-1/A1 functions, similar to Mcl-1, as a selective tBid and Bak antagonist" Oncogene 27(10):1421-1428 (2008). DOI: 10.1038/sj.onc.1210771. PubMed: PMC2880719.
      • Mathew, R et al. "Immortalized mouse epithelial cell models to study the role of apoptosis in cancer" Methods in Enzymology 446:77-106 (2008). DOI: 10.1016/S0076-6879(08)01605-4.
      • Degenhardt, K et al. "Autophagy promotes tumor cell survival and restricts necrosis, inflammation, and tumorigenesis" Cancer Cell 10(1):51-64 (2006). PubMed: 16843265.
      • Kamphorst, JJ et al. "Hypoxic and Ras-transformed cells support growth by scavenging unsaturated fatty acids from lysophospholipids" Proc Natl Acad Sci U S A. 110(22):8882-8887 (2013). DOI: 10.1073/pnas.1307237110. PubMed: PMC3670379.
      • Kamphorst, JJ et al. "Liquid chromatography-high resolution mass spectrometry analysis of fatty acid metabolism" Anal Chem 83(23):9114-9122 (2011). DOI: 10.1021/ac202220b. PubMed: PMC3230881.
      • Fan, J et al. "Glutamine-driven oxidative phosphorylation is a major ATP source in transformed mammalian cells in both normoxia and hypoxia" Mol Syst Biol. 9:712 (2013). DOI: 10.1038/msb.2013.65. PubMed: PMC3882799.
      • Pasternack, RM et al. "Measurement of subcellular texture by optical Gabor-like filtering with a digital micromirror device" Opt Lett. 33(19):2209-2211 (2008). PubMed: PMC2859170.
      • Shimazu, T et al. "NBK/BIK antagonizes MCL-1 and BCL-XL and activates BAK-mediated apoptosis in response to protein synthesis inhibition" Genes Dev. 21(8):929-941 (2007). DOI: 10.1101/gad.1522007. PubMed: PMC1847711.
      • Martino, JJ et al. "Metabotropic glutamate receptor 1 (Grm1) is an oncogene in epithelial cells" Oncogene 32(37):4366-4376 (2013). DOI: 10.1038/onc.2012.471. PubMed: PMC3910169.