BVRA Stable Knockout Hepa1c1c7 Cell Line
|T6282||1x106 cells / 1.0 ml|
|Description||BVRA (biliverdin reductase A) was knocked out of the mouse Hepa1c1c7 cells using sgRNAs inserted into the GuideIt-zsGreen plasmid. The plasmid was transfected and clones were selected via FACS sorting for fluorescence. Knockout was determined by qPCR and Western blot analysis. Studies have shown the loss of BVRA is associated with fat accumulation in livers of mice models. Therefore BVRA Stable Knockout Hepa1c1c7 Cell Line is useful to study the effects of BVRA and its association with obesity increased lipid availability and lipotoxicity in this liver cell line.|
|Species||Mouse (M. musculus)|
|Applications||For Research Use Only|
|Seeding Density||25,000 - 35,000 cells/cm2|
|Population Doubling Time||32 - 42 hours|
|Cell Type||Drug Discovery Cell Lines|
|Expression Profile||Biliverdin reductase A (BVRA) knockout; no drug resistance have been reported|
|Preservation Protocol||1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.|
2. Storage Temperature: Liquid nitrogen vapour phase.
|Unit quantity||1x106 cells / 1.0 ml|
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