BVRA Stable Knockout Hepa1c1c7 Cell Line

T62821x106 cells / 1.0 ml


DescriptionBVRA (biliverdin reductase A) was knocked out of the mouse Hepa1c1c7 cells using sgRNAs inserted into the GuideIt-zsGreen plasmid. The plasmid was transfected and clones were selected via FACS sorting for fluorescence. Knockout was determined by qPCR and Western blot analysis. Studies have shown the loss of BVRA is associated with fat accumulation in livers of mice models. Therefore BVRA Stable Knockout Hepa1c1c7 Cell Line is useful to study the effects of BVRA and its association with obesity increased lipid availability and lipotoxicity in this liver cell line.
SpeciesMouse (M. musculus)
Tissue/Organ/Organ SystemLiver
Cell MorphologyEpithelial-like
ApplicationsFor Research Use Only
Seeding Density25,000 - 35,000 cells/cm2
Population Doubling Time32 - 42 hours
Cell TypeDrug Discovery Cell Lines
Growth PropertiesAdherent
Expression ProfileBiliverdin reductase A (BVRA) knockout; no drug resistance have been reported
Preservation Protocol1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
Unit quantity1x106 cells / 1.0 ml

Supporting Protocol




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