CAP1 Stable Knockdown PANC-1 Cell Line
|T6404||1x106 cells / 1.0 ml|
|Description||CAP1 mediate extracellular signals to control cancer cell invasiveness. Using two shRNA constructs S2 and S3 that target independent nucleotide sequences and effectively silenced CAP1 in HeLa and breast cancer cells we were able to generate stable clones with efficient CAP1 knockdown in the PANC-1 and AsPC-1 cells as confirmed in Western blotting. Knockdown of CAP1 in cancer cells led to enhanced stress fibers as well as reduced cell motility and invasion into Matrigel. These cells can be used to investigated roles for CAP1 and its phosphor-regulation in pancreatic cancer cells.|
|Species||Human (H. sapiens)|
For Research Use Only
|Seeding Density||20,000 - 30,000 cells/cm2|
|Population Doubling Time||31 - 41 hours|
|Donor Disease||Pancreatic cancer|
|Cell Type||Drug Discovery Cell Lines|
PANC-1 (clone S3-3) Drug resistance: 250 ug/ml neomycin Vector information: pRNA-U6.1/Neo vector was used S2 targets nucleotide 519-537 of human CAP1 gene; S3 targets nucleotides 1074-1092 of human CAP1 gene.
|Preservation Protocol||1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.|
2. Storage Temperature: Liquid nitrogen vapour phase.
|Unit quantity||1x106 cells / 1.0 ml|
1) Western blot
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