Cas9 Expressing HT1080 Cell Line

CAT.NOUNITPRICE
T32601x106 cells / 1.0 ml
$795.00

Specifications


Description

HT1080 stably expressing Cas9

SKUT3260
Cas9 ExpressionStably Integrated Cas9
Source OrganConnective Tissue
MorphologyEpithelial
Unit quantity1x106 cells / 1.0 ml
Growth PropertiesAdherent
Propagation
Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.
The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Cells may be grown in the presence of 0.8 µg/ml Puromycin (G264).
Change media every 2-3 days.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
* Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Seeding Density30,000 - 50,000 cells/cm2
Donor Age35 year old
Donor GenderMale
Shipping Conditions

Dry Ice

Quality Control1) Puromycin selection
2) Western Blot analysis for Cas9 transgene expression
Mammalian SelectionPuromycin
Disclaimer

1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.

2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.

6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."

FAQs


What is the PAM sequence required for sgRNA design for use with these cells?
We use spCas9, therefore the PAM is 5'-NGG-3' for this cell line.
Is there a selection marker for cas9 expression?
Yes, Puromycin is selection marker for most of these cell lines. Some cell lines may have additional inherent drug resistance eg. 293T has neomycin resistance. Please refer to the product page for the specific cell line of interest.
References


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