Cas9 Nickase Adenovirus
CAT.NO | UNIT | PRICE |
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K007 | 250 µl | $295.00 |
Description | This Cas9 nickase can be used with two target-specific sgRNAs to bind DNA and create two single-stranded cuts. Due to the requirement for two sgRNA guide sequences to be close together for gene editing to occur, using Cas9 nickase should cause fewer off-target effects than using Cas9 nuclease. |
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SKU | K007 |
System | Adenovirus |
Vector Map | pAdeno-Cas9-Nickase |
Unit quantity | 250 µl |
Titer | 106 pfu/ml |
Promoter | CMV |
Cas Type | Nickase |
Cas Origin | spCas9 |
Cas9 Expression | Constitutive Cas9 |
Shipping Conditions | High titer adenoviruses are shipped with dry ice and low titer adenoviruses are shipped with ice packs. For long term storage, it is recommended to store the viruses at -80°C in small aliquots to avoid repeated freeze-thaw cycles. |
Format | Virus |
Appearance | Liquid |
Quality Control | Restriction Enzyme Digest and Sequencing |
Shelf Life | 1 year (when stored at -20°C or below) |
Caution | This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. |
Supporting Protocol
MSDS
QC
Other
Is this Cas9-nickase codon-optimized for a specific cell type? | |
Codon optimized for human, but not cell or tissue type specific. |
Does this Cas9 nickase Cat# K007 contain any selection marker (fluorescent protein or mammalian toxin resistance like puromycin)? | |
The Cas 9 nickase adenovirus does not contain a mammalian selection marker. |
Is there any tag linked to the enzyme? | |
There is no tag attached to the Cas9. This would be very difficult due to the large insert size. |
There are no references for this product yet!