Cas9 Nickase Adenovirus
| CAT.NO | UNIT | PRICE |
|---|---|---|
| K007 | 250 µl | $295.00 |
| Description | This Cas9 nickase can be used with two target-specific sgRNAs to bind DNA and create two single-stranded cuts. Due to the requirement for two sgRNA guide sequences to be close together for gene editing to occur, using Cas9 nickase should cause fewer off-target effects than using Cas9 nuclease. |
|---|---|
| SKU | K007 |
| System | Adenovirus |
| Vector Map | pAdeno-Cas9-Nickase |
| Unit quantity | 250 µl |
| Titer | 106 pfu/ml |
| Promoter | CMV |
| Cas Type | Nickase |
| Cas Origin | spCas9 |
| Cas9 Expression | Constitutive Cas9 |
| Shipping Conditions | High titer adenoviruses are shipped with dry ice and low titer adenoviruses are shipped with ice packs. For long term storage, it is recommended to store the viruses at -80°C in small aliquots to avoid repeated freeze-thaw cycles. |
| Format | Virus |
| Appearance | Liquid |
| Quality Control | Restriction Enzyme Digest and Sequencing |
| Shelf Life | 1 year (when stored at -20°C or below) |
| Caution | This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. |
Supporting Protocol
MSDS
QC
Other
| Is this Cas9-nickase codon-optimized for a specific cell type? | |
Codon optimized for human, but not cell or tissue type specific. | |
| Does this Cas9 nickase Cat# K007 contain any selection marker (fluorescent protein or mammalian toxin resistance like puromycin)? | |
The Cas 9 nickase adenovirus does not contain a mammalian selection marker. | |
| Is there any tag linked to the enzyme? | |
There is no tag attached to the Cas9. This would be very difficult due to the large insert size. | |
There are no references for this product yet!