Cas9 Nickase Adenovirus

CAT.NOUNITPRICE
K007250 µl
$295.00

Specifications


Description

This Cas9 nickase can be used with two target-specific sgRNAs to bind DNA and create two single-stranded cuts. Due to the requirement for two sgRNA guide sequences to be close together for gene editing to occur, using Cas9 nickase should cause fewer off-target effects than using Cas9 nuclease.

SKUK007
SystemAdenovirus
Vector MappAdeno-Cas9-Nickase
Unit quantity250 µl
Titer106 pfu/ml
PromoterCMV
Cas TypeNickase
Cas OriginspCas9
Cas9 ExpressionConstitutive Cas9
Shipping Conditions

High titer adenoviruses are shipped with dry ice and low titer adenoviruses are shipped with ice packs. For long term storage, it is recommended to store the viruses at -80°C in small aliquots to avoid repeated freeze-thaw cycles.

FormatVirus
AppearanceLiquid
Quality ControlRestriction Enzyme Digest and Sequencing
Shelf Life1 year (when stored at -20°C or below)
CautionThis product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.
Documents


Supporting Protocol

    MSDS

      QC

        Other
        FAQs


        Is this Cas9-nickase codon-optimized for a specific cell type?
        Codon optimized for human, but not cell or tissue type specific.
        Is there any tag linked to the enzyme?
        There is no tag attached to the Cas9. This would be very difficult due to the large insert size.
        References


        There are no references for this product yet!