Cas9 Nickase Lentiviral Vector
This Cas9 nickase can be used with two target-specific sgRNAs to bind DNA and create two single-stranded cuts. Due to the requirement for two sgRNA guide sequences to be close together for gene editing to occur, using Cas9 nickase should cause fewer off-target effects than using Cas9 nuclease.
|Unit quantity||10 µg|
|Cas9 Expression||Constitutive Cas9|
Cas9 Nickase includes N-terminal SV40 NLS
Shipped at ambient temperature
|Quality Control||Restriction Enzyme Digest and Sequencing|
|Shelf Life||1 year (when stored at -20°C or below)|
|Caution||This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.|
|What kind of mutation does this contain?|
|Is this Cas9-nickase codon-optimized for a specific cell type?|
It is codon optimized for human expression.
|What type of selection marker does this contain?|
This lentiviral vector contains a puromycin marker for mammalian selection.
|What is the sequence?|
We are unable to release the sequence of our Cas9 Nickase as it has been codon optimized and is proprietary information.
|Can you provide the sequence for this plasmid?|
We are unable to release the sequence of our Cas9 Nickase / Nuclease as it has been codon optimized for expression and this is proprietary information. A vector map can be located however by clicking on the vector name.
- The Board of Regents of the University of Texas System., . "GENERATION OF GENETICALLY ENGINEERED ANIMALS BY CRISPR/CAS9 GENOME EDITING IN SPERMATOGONIAL STEM CELLS" United States Patent Application 20190134227. Retrieved from : (2019).
- Mohler, . "U" S. Patent Application No. 0201442 A1 : (2019).