Cas9 Nickase Lentiviral Vector

CAT.NOUNITPRICE
K00510 µg
$85.00

Specifications


Description

This Cas9 nickase can be used with two target-specific sgRNAs to bind DNA and create two single-stranded cuts. Due to the requirement for two sgRNA guide sequences to be close together for gene editing to occur, using Cas9 nickase should cause fewer off-target effects than using Cas9 nuclease.

SKUK005
SystemLentiviral Vector
Vector MappLenti-EF1a-Cas9-Nickase-SV40-Puro
Unit quantity10 µg
PromoterEF1a
Cas TypeNickase
Cas OriginspCas9
Cas9 ExpressionConstitutive Cas9
Note

Cas9 Nickase includes N-terminal SV40 NLS

Shipping Conditions

Shipped at ambient temperature

Bacterial SelectionKanamycin
FormatVector
AppearanceLiquid
Quality ControlRestriction Enzyme Digest and Sequencing
Shelf Life1 year (when stored at -20°C or below)
CautionThis product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.
Documents


Supporting Protocol

    MSDS

      QC
      Other

        FAQs


        Is this Cas9-nickase codon-optimized for a specific cell type?
        It is codon optimized for human expression.
        What type of selection marker does this contain?
        This lentiviral vector contains a puromycin marker for mammalian selection.
        What is the sequence?
        We are unable to release the sequence of our Cas9 Nickase as it has been codon optimized and is proprietary information.
        Can you provide the sequence for this plasmid?
        We are unable to release the sequence of our Cas9 Nickase / Nuclease as it has been codon optimized for expression and this is proprietary information. A vector map can be located however by clicking on the vector name.
        References


        2
        • The Board of Regents of the University of Texas System., . "GENERATION OF GENETICALLY ENGINEERED ANIMALS BY CRISPR/CAS9 GENOME EDITING IN SPERMATOGONIAL STEM CELLS" United States Patent Application 20190134227. Retrieved from : (2019).
        • Mohler, . "U" S. Patent Application No. 0201442 A1 : (2019).