Cas9 Nickase Lentiviral Vector
| CAT.NO | UNIT | PRICE |
|---|---|---|
| K005 | 10 µg | $85.00 |
| Description | This Cas9 nickase can be used with two target-specific sgRNAs to bind DNA and create two single-stranded cuts. Due to the requirement for two sgRNA guide sequences to be close together for gene editing to occur, using Cas9 nickase should cause fewer off-target effects than using Cas9 nuclease. |
|---|---|
| SKU | K005 |
| System | Lentiviral Vector |
| Vector Map | pLenti-EF1a-Cas9-Nickase-SV40-Puro |
| Unit quantity | 10 µg |
| Promoter | EF1a |
| Cas Type | Nickase |
| Cas Origin | spCas9 |
| Cas9 Expression | Constitutive Cas9 |
| Note | Cas9 Nickase includes N-terminal SV40 NLS |
| Shipping Conditions | Shipped at ambient temperature |
| Bacterial Selection | Kanamycin |
| Format | Vector |
| Appearance | Liquid |
| Quality Control | Restriction Enzyme Digest and Sequencing |
| Shelf Life | 1 year (when stored at -20°C or below) |
| Caution | This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. |
| What kind of mutation does this contain? | |
D10A mutation | |
| Is this Cas9-nickase codon-optimized for a specific cell type? | |
It is codon optimized for human expression. | |
| What type of selection marker does this contain? | |
This lentiviral vector contains a puromycin marker for mammalian selection. | |
| What is the sequence? | |
We are unable to release the sequence of our Cas9 Nickase as it has been codon optimized and is proprietary information. | |
| Can you provide the sequence for this plasmid? | |
We are unable to release the sequence of our Cas9 Nickase / Nuclease as it has been codon optimized for expression and this is proprietary information. A vector map can be located however by clicking on the vector name.
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2
- The Board of Regents of the University of Texas System., . "GENERATION OF GENETICALLY ENGINEERED ANIMALS BY CRISPR/CAS9 GENOME EDITING IN SPERMATOGONIAL STEM CELLS" United States Patent Application 20190134227. Retrieved from : (2019).
- Mohler, . "U" S. Patent Application No. 0201442 A1 : (2019).