Cas9 Nickase Lentivirus

CAT.NOUNITPRICE
K006300 µl
$295.00

Specifications


Description

This Cas9 nickase can be used with two target-specific sgRNAs to bind DNA and create two single-stranded cuts. Due to the requirement for two sgRNA guide sequences to be close together for gene editing to occur, using Cas9 nickase should cause fewer off-target effects than using Cas9 nuclease.

SKUK006
SystemLentivirus
Vector MappLenti-EF1a-Cas9-Nickase-SV40-Puro
Unit quantity300 µl
Titer107 IU/ml
PromoterEF1a
Cas TypeNickase
Cas OriginspCas9
Cas9 ExpressionConstitutive Cas9
Shipping Conditions

Shipped with dry ice

FormatVirus
AppearanceLiquid
Quality ControlRestriction Enzyme Digest and Sequencing
Shelf Life1 year (when stored at -80°C or below)
CautionThis product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.
Documents


Supporting Protocol

    MSDS

      QC
      Other

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        References


        2
        • The Board of Regents of the University of Texas System., . "GENERATION OF GENETICALLY ENGINEERED ANIMALS BY CRISPR/CAS9 GENOME EDITING IN SPERMATOGONIAL STEM CELLS" United States Patent Application 20190134227. Retrieved from : (2019).
        • Mohler, . "U" S. Patent Application No. 0201442 A1 : (2019).