Cas9 Nickase Lentivirus
This Cas9 nickase can be used with two target-specific sgRNAs to bind DNA and create two single-stranded cuts. Due to the requirement for two sgRNA guide sequences to be close together for gene editing to occur, using Cas9 nickase should cause fewer off-target effects than using Cas9 nuclease.
|Unit quantity||300 µl|
|Cas9 Expression||Constitutive Cas9|
Shipped with dry ice
|Quality Control||Restriction Enzyme Digest and Sequencing|
|Shelf Life||1 year (when stored at -80°C or below)|
|Caution||This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.|
- The Board of Regents of the University of Texas System., . "GENERATION OF GENETICALLY ENGINEERED ANIMALS BY CRISPR/CAS9 GENOME EDITING IN SPERMATOGONIAL STEM CELLS" United States Patent Application 20190134227. Retrieved from : (2019).
- Mohler, . "U" S. Patent Application No. 0201442 A1 : (2019).