Cas9 Nuclease Lentiviral Vector
| CAT.NO | UNIT | PRICE |
|---|---|---|
| K002 | 10 µg | $85.00 |
| Description | This Cas9 nuclease can be used with a target-specific sgRNA to bind DNA and create a double-stranded cut. |
|---|---|
| SKU | K002 |
| System | Lentiviral Vector |
| Vector Map | |
| Unit quantity | 10 µg |
| Promoter | EF1a |
| Cas Type | Nuclease |
| Cas Origin | spCas9 |
| Cas9 Expression | Constitutive Cas9 |
| Note | Cas9 WT includes N-terminal SV40 NLS and C-terminal Nucleoplasmin NLS |
| Shipping Conditions | Shipped at ambient temperature |
| Bacterial Selection | Ampicillin |
| Format | Vector |
| Appearance | Liquid |
| Quality Control | Restriction Enzyme Digest and Sequencing |
| Shelf Life | 1 year (when stored at -20°C or below) |
| Caution | This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. |
Supporting Protocol
MSDS
QC
Other
| I need to design a QPCR primer to detect the expression level of Cas9 in my target cells, can you please provide the Cas9 sequence info? | |
We are unable to supply the full sequence due to propriety reasons. However, we can supply a short ~600-800bp region of the Cas9 sequence for you to design the qPCR primer from. We will be happy to provide this information once an order has been processed. | |
| Can you provide the sequence for this plasmid? | |
We are unable to release the sequence of our Cas9 Nickase / Nuclease as it has been codon optimized for expression and this is proprietary information. A vector map can be located however by clicking on the vector name.
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2
- The Board of Regents of the University of Texas System., . "GENERATION OF GENETICALLY ENGINEERED ANIMALS BY CRISPR/CAS9 GENOME EDITING IN SPERMATOGONIAL STEM CELLS" United States Patent Application 20190134227. Retrieved from : (2019).
- Mohler, . "U" S. Patent Application No. 0201442 A1 : (2019).