Cas9 Nuclease Lentiviral Vector
| Cat. No. | K002 |
| Name | Cas9 Nuclease Lentiviral Vector |
| Unit | 10 µg |
| Description |
Lentiviral Vector expressing Cas9 Nuclease from S. pyogenes with Puromycin resistance. Cas9 includes N-terminal SV40 NLS and C-terminal Nucleoplasmin NLS. |
| Applications |
CRISPR Cas9 gene editing. |
| Storage Condition | -20°C or below. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. K002 |
Print/Download Datasheet
| Do I need to provide my own sgRNA? | |
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Yes, this product only expresses the Cas9 or Cas-related protein. We offer genome-wide sgRNA only expressing vectors for CRISPR gene knockout (spCas9 and saCas9) and CRISPR gene activation and repression which are available through the Customize Vector option. Similarly, abm also offers Custom CRISPR Vectors and Viruses, please inquire with us to get started on your CRISPR project.
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| What is the source of Cas9 in this vector? | |
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The Cas nuclease is the standard spCas9 from Streptococcus pyogenes. It has been codon optimized for mammalian expression.
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- The Board of Regents of the University of Texas System., . "GENERATION OF GENETICALLY ENGINEERED ANIMALS BY CRISPR/CAS9 GENOME EDITING IN SPERMATOGONIAL STEM CELLS" United States Patent Application 20190134227. Retrieved from : (2019).
- Czerkies, M., Kochańczyk, M., Korwek, Z., Prus, W., & Lipniacki, T. (2022). Respiratory Syncytial Virus Protects Bystander Cells against Influenza A Virus Infection by Triggering Secretion of Type I and Type III Interferons. Journal of Virology, 96(22). https://doi.org/10.1128/jvi.01341-22
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