Cas9 Nuclease Lentiviral Vector

CAT.NOUNITPRICE
K00210 µg
$85.00

Specifications


Description

This Cas9 nuclease can be used with a target-specific sgRNA to bind DNA and create a double-stranded cut.

SKUK002
SystemLentiviral Vector
Vector Map

pLenti-EF1a-Cas9-Puro

Unit quantity10 µg
PromoterEF1a
Cas TypeNuclease
Cas OriginspCas9
Cas9 ExpressionConstitutive Cas9
Note

Cas9 WT includes N-terminal SV40 NLS and C-terminal Nucleoplasmin NLS

Shipping Conditions

Shipped at ambient temperature

Bacterial SelectionAmpicillin
FormatVector
AppearanceLiquid
Quality ControlRestriction Enzyme Digest and Sequencing
Shelf Life1 year (when stored at -20°C or below)
CautionThis product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.
Documents


Supporting Protocol

    MSDS

      QC

        Other
        FAQs


        I need to design a QPCR primer to detect the expression level of Cas9 in my target cells, can you please provide the Cas9 sequence info?
        We are unable to supply the full sequence due to propriety reasons. However, we can supply a short ~600-800bp region of the Cas9 sequence for you to design the qPCR primer from. We will be happy to provide this information once an order has been processed.
        Can you provide the sequence for this plasmid?
        We are unable to release the sequence of our Cas9 Nickase / Nuclease as it has been codon optimized for expression and this is proprietary information. A vector map can be located however by clicking on the vector name.
        References


        2
        • The Board of Regents of the University of Texas System., . "GENERATION OF GENETICALLY ENGINEERED ANIMALS BY CRISPR/CAS9 GENOME EDITING IN SPERMATOGONIAL STEM CELLS" United States Patent Application 20190134227. Retrieved from : (2019).
        • Mohler, . "U" S. Patent Application No. 0201442 A1 : (2019).