CEA-2 Stable MC-38 Cell Line
|T6408||1x106 cells / 1.0 ml|
|Description||MC38 murine colonic adenocarcinoma cells were transduced with a retrovirus containing the human Carcinoembryonic antigen (CEA) gene. The CEA gene expressed in T6408 is a single M: 70000 glycosylated immunoreactive product. The CEA gene present in T6408 was partially sequenced and found to have a deletion of two of the three repeated domains contained in CEA. Despite the loss of two domains the CEA product expresses epitopes recognized by multiple anti-CEA MAbs This cell line can be used as a model to study reagents against a carcinoma expressing human CEA.|
|Species||Mouse (M. musculus)|
|Applications||For Research Use Only|
|Donor Disease||Colonic adenocarcinoma|
|Cell Type||Drug Discovery Cell Lines|
|Expression Profile||CEA; G418 resistance|
|Preservation Protocol||1. Freeze Medium: 90% FBS and 10% DMSO.|
2. Storage Temperature: Liquid nitrogen vapour phase.
|Unit quantity||1x106 cells / 1.0 ml|
|QC||1) Western blot; 2) Southern blot; 3) Sequencing; 4) Immunofluorescence assay; 5) Immunoprecipitation|
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