COX10 Stable Knockout Mouse Fibroblast Cell Line

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T63031x106 cells / 1.0 ml
$0.00

Specifications


DescriptionTo generate the COX10 Stable Knockout Mouse Fibroblast Cell Line a primary skin fibroblast culture was established from a knockin mouse homozygous for the floxed COX10 (exon 6). Cells were immortalized by retroviral infection of E6-E7 type 16 papiloma virus carrying G418 resistance. To generate COX10 knock-out; immortalized cells were transfected with plasmid expressing Cre recombinase and clones were obtained after hygromycin selection.
SKUT6303
SpeciesMouse (M. musculus)
Tissue/Organ/Organ SystemSkin
Cell MorphologyFibroblast
ApplicationsFor Research Use Only
Population Doubling Time24 hours
Cell TypeDrug Discovery Cell Lines
Growth PropertiesAdherent
Expression ProfileG418 and hygromycin-resistant
Preservation Protocol1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
Unit quantity1x106 cells / 1.0 ml
QC1) Western blot
Documents


Supporting Protocol
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