COX10 Stable Knockout Mouse Fibroblast Cell Line
|T6303||1x106 cells / 1.0 ml|
|Description||To generate the COX10 Stable Knockout Mouse Fibroblast Cell Line a primary skin fibroblast culture was established from a knockin mouse homozygous for the floxed COX10 (exon 6). Cells were immortalized by retroviral infection of E6-E7 type 16 papiloma virus carrying G418 resistance. To generate COX10 knock-out; immortalized cells were transfected with plasmid expressing Cre recombinase and clones were obtained after hygromycin selection.|
|Species||Mouse (M. musculus)|
|Applications||For Research Use Only|
|Population Doubling Time||24 hours|
|Cell Type||Drug Discovery Cell Lines|
|Expression Profile||G418 and hygromycin-resistant|
|Preservation Protocol||1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.|
2. Storage Temperature: Liquid nitrogen vapour phase.
|Unit quantity||1x106 cells / 1.0 ml|
|QC||1) Western blot|
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