CYP3A5 Point Mutation HuH-7 Cell Line

CAT.NOUNITPRICE
T63481x106 cells / 1.0 ml
$0.00

Specifications


Description

Cells were derived from HuH-7 cells. Cells were genetically modified using CRISPR/Cas9 method with 1 guide RNA and HDR template to heteroygously point mutate CYP3A5 *3 splice junction. Cells have very high CYP3A5 activity whereas the HuH-7 parental cell line had minimal CYP3A5 activity as assessed by tacrolimus and midazolam metabolism assays with or without CYP3A4 selective inhibitor CYP3CIDE.

SKUT6348
SpeciesHuman (H. sapiens)
Tissue/Organ/Organ SystemLiver
Growth PropertiesAdherent
Cell MorphologyEpithelial
Seeding Density17,000 - 34,000 cells/cm2
Population Doubling Time24 - 72 hours
Applications

For Research Use Only

Unit quantity1x106 cells / 1.0 ml
Pharmaceutical TargetEnzymes
Cell TypeDrug Discovery Cell Lines
Propagation Requirements
Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.

The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Antibiotic/Antimycotic (100X) (G273) to a final concentration of 1X.
Change media every 2-3 days.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
* Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Preservation Protocol1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
QC

1) PCR-based genotyping; 2) Quantitative real-time PCR; 3) RNA splicing assay; 4) Immunoblot; 5) Metabolism assays

Disclaimer

1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.

2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.

6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."

DepositorHennepin Healthcare Research Institute
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