Cytochrome c Stable Knockout Mouse Fibroblast Cell Line

T63051x106 cells / 1.0 ml



Mice harboring a heterozygous somatic cyt c KO allele (cyt cs+/-) with mice harboring a homozygous testis cyt c KO allele (cyt ct-/-) were crossed. We used homozygous cyt ct-/- females for the crosses. Embryonic lethal phenotype was rescued by introducing a ubiquitously expressed somatic cyt c transgene flanked by loxP sites. Crosses of one of the transgenic animals with the cyt cyt-/-cyt cs+/- animals gave rise to F1 mice that when backcrossed produced mice with the cyt ct-/-cyt cs-/- cyt cflox/o genotype; demonstrating that the transgene was able to rescue the embryonic lethal phenotype of the cyt cs KO. Lung fibroblasts (LF) from these animals were cultured and the transgenic cyt c cDNA deleted ex vivo by an adenovirus preparation expressing the Cre recombinase. clones by serial dilution and screened them for the presence of Cyt c via immunocytochemistry or western blot in which the resultant clone had no detectable Cyt c to generate the Cytochrome c Stable Knockout Mouse Fibroblast Cell Line.

SpeciesMouse (M. musculus)
Tissue/Organ/Organ SystemLung
Cell MorphologyFibroblast
ApplicationsFor Research Use Only
Population Doubling Time24 hours
Cell TypeDrug Discovery Cell Lines
Growth PropertiesAdherent
Expression ProfileG418 and hygromycin-resistant
Preservation Protocol1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
Unit quantity1x106 cells / 1.0 ml
QC1) Western blot; 2) IHC

Supporting Protocol




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