Cytochrome c Stable Knockout Mouse Fibroblast Cell Line
|T6305||1x106 cells / 1.0 ml|
Mice harboring a heterozygous somatic cyt c KO allele (cyt cs+/-) with mice harboring a homozygous testis cyt c KO allele (cyt ct-/-) were crossed. We used homozygous cyt ct-/- females for the crosses. Embryonic lethal phenotype was rescued by introducing a ubiquitously expressed somatic cyt c transgene flanked by loxP sites. Crosses of one of the transgenic animals with the cyt cyt-/-cyt cs+/- animals gave rise to F1 mice that when backcrossed produced mice with the cyt ct-/-cyt cs-/- cyt cflox/o genotype; demonstrating that the transgene was able to rescue the embryonic lethal phenotype of the cyt cs KO. Lung fibroblasts (LF) from these animals were cultured and the transgenic cyt c cDNA deleted ex vivo by an adenovirus preparation expressing the Cre recombinase. clones by serial dilution and screened them for the presence of Cyt c via immunocytochemistry or western blot in which the resultant clone had no detectable Cyt c to generate the Cytochrome c Stable Knockout Mouse Fibroblast Cell Line.
|Species||Mouse (M. musculus)|
|Applications||For Research Use Only|
|Population Doubling Time||24 hours|
|Cell Type||Drug Discovery Cell Lines|
|Expression Profile||G418 and hygromycin-resistant|
|Preservation Protocol||1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.|
2. Storage Temperature: Liquid nitrogen vapour phase.
|Unit quantity||1x106 cells / 1.0 ml|
|QC||1) Western blot; 2) IHC|
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