dCas9-TET1CD Lentiviral Vector

CAT.NOUNITPRICE
K09610 µg
$395.00

Specifications


Description

This construct consists of the catalytic domain of Ten-Eleven Translocation dioxygenase 1 (TET1CD) fused to the C-terminus of Cas9 Double Mutant (dCas9). The Cas9 Double Mutant has changes at amino acid positions D10A and H840A which completely inactivate both nuclease and nickase activities; whereas the TET1 domain facilitates the process of demethylation leading to transcriptional up-regulation. By using specific sgRNAs, the dCas9-TET1CD can be targeted to a promoter region allowing for epigenetic control over the expression of almost any gene of interest.

SKUK096
SystemLentiviral Vector
Product Applications
  • Direct plasmid transfection for immediate expression
  • Package into Lentiviral particles for high efficiency transduction and stably integrated expression.
Vector Map

pLenti-EF1a-dCas9-TET1CD

Unit quantity10 µg
PromoterEF1a
Cas TypeNull Mutant
Cas OriginspCas9
Cas9 ExpressionConstitutive Cas9
Note

abm's sgRNAs are designed for use with Cas9 Nuclease only. Please inquire for custom sgRNAs for purposes other than genome editing (e.g. CRISPRa). For CRISPR-Cas9 system applications in genome regulation, please see our comprehensive collection of Cas9 mutant proteins (Nickase, Null mutant).

Shipping Conditions

Shipped at ambient temperature

Bacterial SelectionKanamycin
FormatPlasmid
AppearanceLiquid
Quality ControlRestriction Enzyme Digest and Sequencing
Shelf Life1 year (when at -20°C or below in a non-frost free freezer)
CautionThis product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.
Documents


Supporting Protocol

    MSDS

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