dCas9-TET1CD Lentiviral Vector
| CAT.NO | UNIT | PRICE |
|---|---|---|
| K096 | 10 µg | $395.00 |
| Description | This construct consists of the catalytic domain of Ten-Eleven Translocation dioxygenase 1 (TET1CD) fused to the C-terminus of Cas9 Double Mutant (dCas9). The Cas9 Double Mutant has changes at amino acid positions D10A and H840A which completely inactivate both nuclease and nickase activities; whereas the TET1 domain facilitates the process of demethylation leading to transcriptional up-regulation. By using specific sgRNAs, the dCas9-TET1CD can be targeted to a promoter region allowing for epigenetic control over the expression of almost any gene of interest. |
|---|---|
| SKU | K096 |
| System | Lentiviral Vector |
| Product Applications |
|
| Vector Map | |
| Unit quantity | 10 µg |
| Promoter | EF1a |
| Cas Type | Null Mutant |
| Cas Origin | spCas9 |
| Cas9 Expression | Constitutive Cas9 |
| Note | abm's sgRNAs are designed for use with Cas9 Nuclease only. Please inquire for custom sgRNAs for purposes other than genome editing (e.g. CRISPRa). For CRISPR-Cas9 system applications in genome regulation, please see our comprehensive collection of Cas9 mutant proteins (Nickase, Null mutant). |
| Shipping Conditions | Shipped at ambient temperature |
| Bacterial Selection | Kanamycin |
| Format | Plasmid |
| Appearance | Liquid |
| Quality Control | Restriction Enzyme Digest and Sequencing |
| Shelf Life | 1 year (when at -20°C or below in a non-frost free freezer) |
| Caution | This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. |
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