DNase I (RNase-Free)

Cat. No.
E091
Unit
200 U (100 μl)
Price
$99.00
Cat. No. E091
Name DNase I (RNase-Free)
Unit 200 U (100 μl)
Category Molecular Biology Enzymes and Kits
Description

DNase I is a non-specific endonuclease derived from bovine pancreas that catalyzes the cleavage of phosphodiester bonds in single/double-stranded DNA, chromatin, and RNA:DNA hybrids to generate di-and/or oligonucleotide (5’-phosphorylated and 3’-hydroxylated) end-products. This product is RNase free and can be used in RNA applications.

Product Component Quantity
DNase I (RNase-Free) 200 U (100 μl)
10X DNase I Reaction Buffer 1.0 ml
Application

• Removes DNA from protein preparations and RNA samples
• Mediates nick translation
• Generates random fragments for dideoxy sequencing
• Degrade template DNA following in vitro transcription
• Mediate DNase I foot-printing

Concentration 2 U/μl
Note

One unit is defined as the amount of DNase I that catalyzes the degradation of 1 μg of DNA in 10 minutes at 37 °C into tetranucleotides or smaller fragments.

This product  should not be used in digestions longer than 15 minutes or at temperatures higher than 37°C. For non-RNAse-Free DNAse I, see Cat. No. G028.

This product is distributed for laboratory research only

Caution: Not for diagnostic use.

Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. E091
Datasheet
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