DNase I (RNase-Free)

CAT.NOUNITPRICE
E091200 U (100 μl)
$95.00

Customize DNase I (RNase-Free)
1 x DNase I (RNase-Free)   + $0.00
1 x 10X DNase I Reaction Buffer   + $0.00

* Required Fields

Your Customization
DNase I (RNase-Free)
DNase I (RNase-Free)

In stock

$95.00

Summary
    Specifications


    Description

    DNase I is a non-specific endonuclease derived from bovine pancreas that catalyzes the cleavage of phosphodiester bonds in single/double-stranded DNA, chromatin, and RNA:DNA hybrids to generate di-and/or oligonucleotide (5’-phosphorylated and 3’-hydroxylated) end-products. This product is RNase free and can be used in RNA applications.

    Product ComponentQuantity
    DNase I (RNase-Free) 200 U (100 μl)
    10X DNase I Reaction Buffer 1.0 ml
    SKUE091
    Unit quantity200 U (100 μl)
    Applications

    • Removes DNA from protein preparations and RNA samples
    • Mediates nick translation
    • Generates random fragments for dideoxy sequencing
    • Degrade template DNA following in vitro transcription
    • Mediate DNase I foot-printing

    Concentration2 U/μl
    Note

    One unit is defined as the amount of DNase I that catalyzes the degradation of 1 μg of DNA in 10 minutes at 37 °C into tetranucleotides or smaller fragments.

    This product  should not be used in digestions longer than 15 minutes or at temperatures higher than 37°C. For non-RNAse-Free DNAse I, see Cat. No. G028.

    This product is distributed for laboratory research only

    Caution: Not for diagnostic use.

    Documents


    Supporting Protocol
    MSDS

      QC

        Other

          FAQs


          There are no FAQs for this product yet!
          References


          There are no references for this product yet!