DNase I (RNAse-Free)

CAT.NOUNITPRICE
E091200 U (100 μl)
$95.00

Customize DNase I (RNAse-Free)
1 x DNaseI   + $0.00
1 x 10X DNaseI Rxn Buffer   + $0.00

* Required Fields

Your Customization
DNase I (RNAse-Free)
DNase I (RNAse-Free)

In stock

$95.00

Summary
    Specifications


    DescriptionDeoxyribonuclease I (DNase I; EC 3.1.21.1) is a non-specific endonuclease that catalyzes the cleavage of phosphodiester bonds in single/double-stranded DNA, chromatin, and RNA-DNA hybrids. DNase I cleaves DNA to release di-and/or oligonucleotide (5’-phosphorylated and 3’-hydroxylated) end-products. This product can be used for RNA applications.
    SKUE091
    Unit quantity200 U (100 μl)
    Applications

    • Removes DNA from protein preparations and RNA samples
    • Mediates nick translation
    • Generates random fragments for dideoxy sequencing
    • Degrade template DNA following in vitro transcription
    • Mediate DNase I foot-printing

    Concentration2 U/μl
    Note

    One unit is defined as the amount of DNase I that catalyzes the degradation of 1 μg of DNA in 10 minutes at 37 °C into tetranucleotides or smaller fragments.

    This product does not contain any RNase inhibitors, so it is recommended to be used in combination with RNaseOFF Ribonuclease Inhibitor (Cat. G138). This product should not be used in digestions longer than 15 minutes or at temperatures higher than 37°C, or the residual RNase activity will begin to degrade the RNA.

    This product is distributed for laboratory research only

    Caution: Not for diagnostic use.

    Documents


    Supporting Protocol

      MSDS

        QC

          Other

            FAQs


            There are no FAQs for this product yet!
            References


            There are no references for this product yet!