Dual Notch1-YFP and Inducible Delta-mCherry Reporter CHO-K1 (hN1) Cell Line

T30321x106 cells / 1.0 ml



The Notch-Delta signaling pathway plays a vital role in determining cell fates by mediating communication between neighboring cells during development. The Delta ligand has two important roles: it trans-activates Notch in neighboring cells, and cis-inhibits Notch in its own cell. Recent studies suggest that inhibitory same-cell interaction between the Notch receptor and its Delta ligand have significant consequences in the modes of intercellular signaling.
The dual reporter cell lines can monitor the transcriptional response of Notch signaling activity owing to its stable Notch receptor expression and corresponding citrine fluorescent protein (YFP) reporter. In addition, the dual reporter cell lines have doxycycline-inducible chimeric Delta (rDII1)-mCherry fusion transmembrane protein, making these cell lines useful as a quantitative platform for analysing gene regulatory circuits in living cells and for better understanding of the network and signaling dynamics in the Notch-Delta system. The hN1 cell line stably expresses the human full length Notch1 gene while the hN1G4esn has an intracellular domain of hNotch1 that is replaced with a minimal variant of the transcriptional activator Gal4 to avoid activation of endogenous Notch targets (replacing amino acids 1742 to 2556 of hNotch1 with the amino acids 1-147 and 768-881 of Gal4). hN1 developed through transfection with plasmids: pcDNA5‐TO‐Dl‐mCherry and pCS‐H2B‐Cerulean. Both cell lines show no detectable endogenous Notch or Delta activities and can be induced with 100 ng/ml doxycycline to express Delta-mCherry.

SpeciesChinese Hamster (C. griseus)
Tissue/Organ/Organ SystemOvary
Growth PropertiesAdherent
Cell MorphologyEpithelial-like
Seeding Density20,000 - 40,000 cells/cm2
Population Doubling Time20 - 30 hours

For Research Use Only

Unit quantity1x106 cells / 1.0 ml
Pharmaceutical TargetOther
Cell TypeDrug Discovery Cells
Propagation Requirements
Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.
The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
Preservation Protocol1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

1) qRT-PCR was used to assess the expression of Notch mRNA levels in the hN1G4esn and hN1 cells and compared to early T-cell progenitors; 2) Notch activity induced YFP signal was assessed using IgG-Deltaext fusion proteins fixed to plates at different concentrations and time-lapse recording


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2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

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7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."


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  • Sprinzak , D et al. "Cis-interactions between Notch and Delta generate mutually exclusive signalling states" Nature 465(7294):86–90 (2010). PubMed: 20418862.
  • LeBon, L et al. "Fringe proteins modulate Notch-ligand cis and trans interactions to specify signaling states" Elife Sep 25:3 (2014). PubMed: 25255098.