EGFR-K721A Stable Expressing CHO Cell Line
|T6256||1x106 cells / 1.0 ml|
CHO cells were transfected with a plasmid carrying N1-EGFR(K721A)-YFP to establish the EGFR-K721A Stable CHO Cell Line. The cells were selected with 800 ug/ml G418 then sorted through FACS for fluorescence-positive cells. EGFR-K721A is EGFR mutated to have an inactive tyrosine kinase domain. May be used alongside with EGFR Stable CHO Cell Line (abm; Cat. No. T6255) to study EGFR.
|Species||Chinese Hamster (C. griseus)|
For Research Use Only
|Seeding Density||20,000 - 40,000 cells/cm2|
|Population Doubling Time||20 - 28 hours|
|Cell Type||Drug Discovery Cell Lines|
YFP; G418 resistance; EGFR K721A mutant
|Preservation Protocol||1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.|
2. Storage Temperature: Liquid nitrogen vapour phase.
|Unit quantity||1x106 cells / 1.0 ml|
1) FACS sorting
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