EGFR-K721A Stable Expressing CHO Cell Line

CAT.NOUNITPRICE
T62561x106 cells / 1.0 ml
$0.00

Specifications


Description

CHO cells were transfected with a plasmid carrying N1-EGFR(K721A)-YFP to establish the EGFR-K721A Stable CHO Cell Line. The cells were selected with 800 ug/ml G418 then sorted through FACS for fluorescence-positive cells. EGFR-K721A is EGFR mutated to have an inactive tyrosine kinase domain. May be used alongside with EGFR Stable CHO Cell Line (abm; Cat. No. T6255) to study EGFR.

SKUT6256
SpeciesChinese Hamster (C. griseus)
Tissue/Organ/Organ SystemOvary
Cell MorphologyEpithelial-like
Applications

For Research Use Only

Seeding Density20,000 - 40,000 cells/cm2
Population Doubling Time20 - 28 hours
Cell TypeDrug Discovery Cell Lines
Growth PropertiesAdherent
Expression Profile

YFP; G418 resistance; EGFR K721A mutant

Preservation Protocol1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
Unit quantity1x106 cells / 1.0 ml
QC

1) FACS sorting

Documents


Supporting Protocol
MSDS

    QC

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