FGFR1 Stable U2OS Cell Line

CAT.NOUNITPRICE
T60171x106 cells / 1.0 ml
$0.00

Specifications


Description

FGFR1 Stable U2OS Cell Line can be used as a research tool to investigate ubiquitination of FGFR1, effects with FGF1 stimulation, and pathways involved. Parental U2OS cells were stably transfected with plasmid carrying the human FGFR1 gene. The cells are desirable for further manipulation and used to study the effects.

SKUT6017
SpeciesHuman (H. sapiens)
Tissue/Organ/Organ SystemSkeletal
Donor GenderFemale
Donor Age15 year old
Donor EthnicityCaucasian
Growth PropertiesAdherent
Cell MorphologyEpithelial-like
Seeding Density20,000 - 50,000 cells/cm2; Split ratio: 1:3
Population Doubling Time130 - 140 hours
Applications

For Research Use Only

Unit quantity1x106 cells / 1.0 ml
Pharmaceutical TargetKinases
CautionFor Research Use Only
Cell TypeDrug Discovery Cells
Propagation Requirements
Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.

The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, G418 (G271) to a final concentration of 1 mg/ml and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
* Do not use heat-inactivated FBS for cell culture unless specified otherwise.

QC

1) Selection with 1 mg/ml G418 in culture; 2) receptor expression level analyzed by immunofluorescence and immunoblotting

Disclaimer

1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.

2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.

6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."

DepositorInven2
Documents


Supporting Protocol
MSDS

    QC

      Other

      FAQs


      There are no FAQs for this product yet!
      References


      3
      • Haugsten, EM et al. "Ubiquitination of Fibroblast Growth Factor Receptor 1 Is Required for Its Intracellular Sorting but Not for Its Endocytosis" Mol Biol Cell 19(8):3390–3403 (2008). DOI: 10.1091/mbc.E07-12-1219.
      • Zhen, Y et al. "Nuclear Import of Exogenous FGF1 Requires the ER-Protein LRRC59 and the Importins Kpnα1 and Kpnβ1" Traffic 13(5):650-664 (2012). DOI: 10.1111/j.1600-0854.2012.01341.x.
      • Zakrzewska, M et al. "Increased Protein Stability of FGF1 Can Compensate for Its Reduced Affinity for Heparin" Journal of Biological Chemistry 284(37):25388-25403 (2009). DOI: 10.1074/jbc.m109.001289.