GALE Stable Knockout HEK293T Cell Line (Clone 4)

T61501x106 cells / 1.0 ml



The GALE gene encodes UDP-galactose-4 epimerase which operates in the Leloir pathway, the primary pathway for galactose metabolism. This pathway is critical for the generation of UDP-sugars and catalyzes UDP-glucose to UDP-galactose and UDP-N acetylgalactosamine to UDP N-acetylgalactosamine which serve as precursors for O-linked glycosylation and as the major metabolism pathway for galactose. As this cell line is derived from a human source, it may be useful in the study of glycosylation and how it is affected during instances of disease.
GALE Stable Knockout HEK293T Cell Line (Clone 4) was generated through the knockout of the GALE gene in HEK293T cells using the CRISPR-Cas 9 system to produce a cell line that is deficient in O-glycans. Three separate Guide RNAs were cloned into an expression vector containing a GFP tag which was then transiently transfected into HEK293T cells. Successfully transfected cells were screened for GFP expression and the resulting clones sequenced for GALE. O-glycosolyation levels in this line can be restored with the addition of GalNac and Galactose to the media.

SpeciesHuman (H. sapiens)
Tissue/Organ/Organ SystemKidney
Growth PropertiesAdherent
Cell MorphologyEpithelial
Seeding Density20,000 - 40,000 cells/cm2
Population Doubling Time24 - 34 hours

For Research Use Only

Unit quantity1x106 cells / 1.0 ml
Pharmaceutical TargetEnzymes
Cell TypeDrug Discovery Cell Lines
Propagation Requirements
Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.

The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
* Do not use heat-inactivated FBS for cell culture unless specified otherwise.


1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.

2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.

6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."

DepositorUniversity of Miami

Supporting Protocol




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